Cdk9-55: A new player in muscle regeneration

Cristina Giacinti, Antonio Musarò, Giulia De Falco, Isabelle Jourdan, Mario Molinaro, Luigi Bagella, Cristiano Simone, Antonio Giordano

Research output: Contribution to journalArticlepeer-review

Abstract

Adult skeletal muscle contains a specialized population of myogenic quiescent stem cells, termed satellite cells, which contribute to repair myofibers after injury. During muscle regeneration, satellite cells exit their normal quiescent state, proliferate, activating MyoD and Myf-5 expression, and finally differentiate and fuse to reconstitute the injured muscle architecture. We have previously reported that cdk9 is required for myogenesis in vitro by activating MyoD-dependent transcription. In myoblasts induced to differentiate, HyoD recruits cdk9 on the chromatin of muscle-specific regulatory regions. This event correlates with chromatin-modifying enzyme recruitment and phosphorylation of cdk9-specific target residues at the carboxyl-terminal domain of RNA polymerase II. Here we report that a second cdk9 isoform, termed cdk9-55, plays a fundamental role in muscle regeneration and differentiation in vivo. This alternative form is specifically induced in injured myofibers and its activity is strictly required for the completion of muscle regeneration process.

Original languageEnglish
Pages (from-to)576-582
Number of pages7
JournalJournal of Cellular Physiology
Volume216
Issue number3
DOIs
Publication statusPublished - Sep 2008

ASJC Scopus subject areas

  • Clinical Biochemistry
  • Cell Biology
  • Physiology

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