cDNA cloning, characterization and chromosome mapping of Crtap encoding the mouse cartilage associated protein

Roy Morello, Laura Tonachini, Massimiliano Monticone, Luigi Viggiano, Mariano Rocchi, Ranieri Cancedda, Patrizio Castagnola

Research output: Contribution to journalArticle

Abstract

Recently we have isolated and characterized a cDNA coding for a novel developmentally regulated chick embryo protein, cartilage associated protein (GASP). Here we describe the isolation and characterization of the cDNAs coding for the mouse GASP. Comparison of the mammalian putative protein sequence with the chick sequence shows a very high identity overall (51%); in particular the chick protein is homologous to the half amino terminus of the mouse protein. Furthermore, the comparison of the GASP cDNA sequence with sequences of the genebank database confirms our hypothesis that the GASP genes belong to a novel family that also includes genes encoding for some nuclear antigens. In all mouse tissues examined three GASP mRNAs species are detected, whereas in chick tissues a single mRNA is present. Immunohistochemistry studies show that the protein is expressed in all mouse embryonic cartilages. The mouse cartilage associated protein gene (Crtap) was assigned to chromosome 9F3-F4 by fluorescence in situ hybridization.

Original languageEnglish
Pages (from-to)319-324
Number of pages6
JournalMatrix Biology
Volume18
Issue number3
DOIs
Publication statusPublished - Jun 1 1999

Keywords

  • Cartilage
  • Chondrocyte differentiation
  • Extracellular matrix
  • Gene mapping

ASJC Scopus subject areas

  • Molecular Biology

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