CDNA cloning, characterization and chromosome mapping of the gene encoding human cartilage associated protein (CRTAP)

L. Tonachini, R. Morello, M. Monticone, J. Skaug, S. W. Scherer, R. Cancedda, Patrizio Castagnola

Research output: Contribution to journalArticlepeer-review

Abstract

We have recently isolated and characterized cDNA clones coding for a novel developmentally regulated avian and mouse embryo protein, CASP for Cartilage Associated Protein. Here we describe the isolation and characterization of the gene coding for the human CASP. The comparison of the putative human and mouse protein sequences with the chick sequence revealed an overall high identity (89% and 51%, respectively). Homology search with known DNA and protein sequences showed that CASPs are related to two mammalian nuclear proteins. Here we demonstrate definitively that CASPs are distinct from these nuclear proteins. However, sequence comparison analyses suggest that all of these proteins belong to a new family. In all human tissues examined two CASP mRNA species were detected, whereas a single mRNA and three mRNAs were found in chick and mouse, respectively. The human CASP gene (CRTAP) was assigned to chromosome 3p22 by fluorescence in situ hybridization. Copyright (C) 2000 S.Karger AG, Basel.

Original languageEnglish
Pages (from-to)191-194
Number of pages4
JournalCytogenetics and Cell Genetics
Volume87
Issue number3-4
Publication statusPublished - 1999

ASJC Scopus subject areas

  • Genetics
  • Cell Biology

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