cDNA cloning of turtle prion protein

Tatjana Simonic, Stefano Duga, Bice Strumbo, Rosanna Asselta, Fabrizio Ceciliani, Severino Ronchi

Research output: Contribution to journalArticle

82 Citations (Scopus)

Abstract

Cloning of the cDNA coding for the 270-residue turtle prion protein is reported. It represents the most remote example thus far described. The entire coding region is comprised in a single exon, while a large intron interrupts the 5' UTR. The common structural features of the known prion proteins are all conserved in turtle PrP, whose identity degree to mammalian and avian proteins is about 40 and 58%, respectively. The most intriguing feature, unique to the turtle prion, is the presence of an EF-hand Ca2+ binding motif in the C-terminal half of the protein. Copyright (C) 2000 Federation of European Biochemical Societies.

Original languageEnglish
Pages (from-to)33-38
Number of pages6
JournalFEBS Letters
Volume469
Issue number1
DOIs
Publication statusPublished - Mar 3 2000

Fingerprint

Turtles
Cloning
Organism Cloning
Avian Proteins
Complementary DNA
Prions
5' Untranslated Regions
Introns
EF Hand Motifs
Exons
Proteins
Prion Proteins

Keywords

  • cDNA cloning
  • EF-hand Ca binding motif
  • Gene organization
  • Reptile prion protein

ASJC Scopus subject areas

  • Biochemistry
  • Biophysics
  • Molecular Biology

Cite this

Simonic, T., Duga, S., Strumbo, B., Asselta, R., Ceciliani, F., & Ronchi, S. (2000). cDNA cloning of turtle prion protein. FEBS Letters, 469(1), 33-38. https://doi.org/10.1016/S0014-5793(00)01232-1

cDNA cloning of turtle prion protein. / Simonic, Tatjana; Duga, Stefano; Strumbo, Bice; Asselta, Rosanna; Ceciliani, Fabrizio; Ronchi, Severino.

In: FEBS Letters, Vol. 469, No. 1, 03.03.2000, p. 33-38.

Research output: Contribution to journalArticle

Simonic, T, Duga, S, Strumbo, B, Asselta, R, Ceciliani, F & Ronchi, S 2000, 'cDNA cloning of turtle prion protein', FEBS Letters, vol. 469, no. 1, pp. 33-38. https://doi.org/10.1016/S0014-5793(00)01232-1
Simonic, Tatjana ; Duga, Stefano ; Strumbo, Bice ; Asselta, Rosanna ; Ceciliani, Fabrizio ; Ronchi, Severino. / cDNA cloning of turtle prion protein. In: FEBS Letters. 2000 ; Vol. 469, No. 1. pp. 33-38.
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