cDNA isolation, expression analysis, and chromosomal localization of two human zinc finger genes

Luigi Lania, Emilio Donti, Antonio Pannuti, Anna Pascucci, Gina Pengue, Isidoro Feliciello, Girolama La Mantia, Luisa Lanfrancone, Pier Giuseppe Pelicci

Research output: Contribution to journalArticlepeer-review

Abstract

On the basis of sequence similarity in the repeated zinc finger domain, we have identified and characterized two human cDNA clones (ZNF7 and ZNF8), both encoding proteins containing potential finger-like nucleic acid binding motifs. Northern blot analysis indicates that both genes are expressed as multiple transcripts and they are ubiquitously present in many human cell lines of different embryological derivation. Moreover, their expression is modulated during in vitro induced terminal differentiation of human myeloid cell line HL-60. By in situ hybridization experiments, we have localized the ZNF7 gene to chromosome 8 (region q24) and the ZNF8 gene to the terminal band of the long arm of chromosome 20 (20q13).

Original languageEnglish
Pages (from-to)333-340
Number of pages8
JournalGenomics
Volume6
Issue number2
DOIs
Publication statusPublished - 1990

ASJC Scopus subject areas

  • Genetics

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