CEA and NCA expressed by colon carcinoma cells affect their interaction with and lysability by activated lymphocytes

L. Rivoltini, G. Cattoretti, F. Arienti, A. Mastroianni, G. Parmiani

Research output: Contribution to journalArticlepeer-review

Abstract

Heterogeneous lysability by interleukin-2 activated lymphocytes (LAK) and other immune effectors was observed in the human colon-carcinoma lines LoVo/Dx, LoVo/H and HP29. The tumor cells with high susceptibility to LAK (LoVo/Dx, HL29) expressed higher amounts of the adhesion molecules ICAMl, LFA3 and NCA/CEA than cells with low LAK sensitivity (LoVo/H). Monoclonal antibodies against these molecules caused a marked reduction of lysis by LAK of LoVo/Dx and HT29. A pool of these antibodies induced a nearly complete inhibition of the LAK lysis of both lines. Treatment of LoVo/Dx with differentiating agents (dimethylformamide and retinoic acid) led to a decreased expression of the adhesion molecules, including NCA, accompanied by increased resistance to LAK-mediated lysis. Moreover, the presence of CEA soluble antigen drastically inhibited the cytotoxic activity of LAK effectors against HL29 and LoVo/Dx cells, in a dose-dependent manner. These data indicate that sensitivity of colon-carcinoma cells to activated lymphocytes depends on the level of expression of adhesion molecules, including CEA and NCA. Given the role of CEA-related antigens in tumor/lymphocyte interaction, soluble CEA, frequently released by colon-carcinoma, may be involved in immunosuppressive effects induced in vivo by tumor cells.

Original languageEnglish
Pages (from-to)143-147
Number of pages5
JournalInternational Journal of Biological Markers
Volume7
Issue number3
Publication statusPublished - 1992

ASJC Scopus subject areas

  • Biochemistry
  • Immunology

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