Cefixime and ceftriaxone susceptibility of Neisseria gonorrhoeae in Italy from 2006 to 2010

A. Carannante, G. Prignano, M. Cusini, A. Matteelli, I. Dal Conte, V. Ghisetti, A. D'Antuono, F. Cavrini, R. Antonetti, P. Stefanelli

Research output: Contribution to journalArticle

16 Citations (Scopus)

Abstract

Neisseria gonorrhoeae resistance to cephalosporins, the currently recommended treatment, and treatment failures with cefixime have been reported worldwide. The purposes of the present study were (i) to examine the susceptibility of N. gonorrhoeae isolates isolated in Italy from 2006 through 2010 to cefixime (n=293) taking into account both European Committee on Antimicrobial Susceptibility Testing (EUCAST) and Clinical And Laboratory Standards Institute (CLSI) criteria for categorization; (ii) to determine the contribution to decreased/resistant susceptibility of mutations in the penA, mtrR, ponA and porB1b genes in a subsample of isolates; and (iii) to genotype the isolates showing decreased susceptibility or resistance to cefixime, by N. gonorrhoeae multi-antigen sequence typing (NG-MAST) and by pulsed-field gel electrophoresis (PFGE) to identify the predominant genotypes. Minimum inhibitory concentrations (MICs) were determined by the E-test and agar dilution method on 293 isolates and results were interpreted according to both EUCAST 2010 (MIC R>0.12mg/L) and CLSI 2008 (MIC R>0.25mg/L) criteria. All isolates showed full susceptibility to ceftriaxone, whereas those with a MIC for cefixime ≥0.125mg/L were on the increase from 2008 through 2010. The same penA gene alterations were found among isolates with MICs close to the EUCAST breakpoint as the resistant ones, and they belong to ST1407. Seven isolates, belonging to various sequence types, showed a different por allele, though similar to the por 908 allele present in ST1407. PFGE divided strains ST1407 into two main groups confirming their genetic relationship.

Original languageEnglish
Pages (from-to)558-564
Number of pages7
JournalClinical Microbiology and Infection
Volume18
Issue number6
DOIs
Publication statusPublished - Jun 2012

Fingerprint

Cefixime
Ceftriaxone
Neisseria gonorrhoeae
Microbial Sensitivity Tests
Italy
Pulsed Field Gel Electrophoresis
Cephalosporin Resistance
Alleles
Genotype
Treatment Failure
Genes
Agar
Antigens
Mutation

Keywords

  • Cefixime
  • Ceftriaxone
  • Neisseria gonorrhoeae
  • Pena gene
  • Resistance

ASJC Scopus subject areas

  • Microbiology (medical)
  • Infectious Diseases

Cite this

Cefixime and ceftriaxone susceptibility of Neisseria gonorrhoeae in Italy from 2006 to 2010. / Carannante, A.; Prignano, G.; Cusini, M.; Matteelli, A.; Dal Conte, I.; Ghisetti, V.; D'Antuono, A.; Cavrini, F.; Antonetti, R.; Stefanelli, P.

In: Clinical Microbiology and Infection, Vol. 18, No. 6, 06.2012, p. 558-564.

Research output: Contribution to journalArticle

Carannante, A, Prignano, G, Cusini, M, Matteelli, A, Dal Conte, I, Ghisetti, V, D'Antuono, A, Cavrini, F, Antonetti, R & Stefanelli, P 2012, 'Cefixime and ceftriaxone susceptibility of Neisseria gonorrhoeae in Italy from 2006 to 2010', Clinical Microbiology and Infection, vol. 18, no. 6, pp. 558-564. https://doi.org/10.1111/j.1469-0691.2011.03619.x
Carannante, A. ; Prignano, G. ; Cusini, M. ; Matteelli, A. ; Dal Conte, I. ; Ghisetti, V. ; D'Antuono, A. ; Cavrini, F. ; Antonetti, R. ; Stefanelli, P. / Cefixime and ceftriaxone susceptibility of Neisseria gonorrhoeae in Italy from 2006 to 2010. In: Clinical Microbiology and Infection. 2012 ; Vol. 18, No. 6. pp. 558-564.
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