TY - JOUR
T1 - Cell age-related monovalent cations content and density changes in stored human erythrocytes
AU - Minetti, Giampaolo
AU - Ciana, Annarita
AU - Profumo, Antonella
AU - Zappa, Manuela
AU - Vercellati, Cristina
AU - Zanella, Alberto
AU - Arduini, Arduino
AU - Brovelli, Augusta
PY - 2001/8/15
Y1 - 2001/8/15
N2 - Conversion of erythrocyte membrane protein 4.1b to 4.1a occurs through a non-enzymatic deamidation reaction in most mammalian erythrocytes, with an in vivo half-life of approximately 41 days, making the 4.1a/4.1b ratio a useful index of red cell age [Inaba and Maede, Biochim. Biophys. Acta 944 (1988) 256-264]. Normal human erythrocytes distribute into subpopulations of increasing cell density and cell age when centrifuged in polyarabinogalactan density gradients. We have observed that, when erythrocytes were stored at 4°C under standard blood bank conditions, the deamidation was virtually undetectable, as cells maintained the 4.1a/4.1b ratio they displayed at the onset of storage. By measuring the 4.1a/4.1b values in subpopulations of cells of different density at various time points during storage, a modification of the normal 'cell age/cell density'] relationship was observed, as erythrocytes were affected by changes in cell volume in an age-dependent manner. This may stem from a different impact of storage on the imbalance of monovalent cations, Na+ and K+, in young and old erythrocytes, related to their different complement of cation transporters.
AB - Conversion of erythrocyte membrane protein 4.1b to 4.1a occurs through a non-enzymatic deamidation reaction in most mammalian erythrocytes, with an in vivo half-life of approximately 41 days, making the 4.1a/4.1b ratio a useful index of red cell age [Inaba and Maede, Biochim. Biophys. Acta 944 (1988) 256-264]. Normal human erythrocytes distribute into subpopulations of increasing cell density and cell age when centrifuged in polyarabinogalactan density gradients. We have observed that, when erythrocytes were stored at 4°C under standard blood bank conditions, the deamidation was virtually undetectable, as cells maintained the 4.1a/4.1b ratio they displayed at the onset of storage. By measuring the 4.1a/4.1b values in subpopulations of cells of different density at various time points during storage, a modification of the normal 'cell age/cell density'] relationship was observed, as erythrocytes were affected by changes in cell volume in an age-dependent manner. This may stem from a different impact of storage on the imbalance of monovalent cations, Na+ and K+, in young and old erythrocytes, related to their different complement of cation transporters.
KW - Cell age
KW - Human erythrocyte
KW - Monovalent cation
KW - Protein 4.1a
KW - Protein 4.1b
KW - Storage
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U2 - 10.1016/S0304-4165(01)00159-3
DO - 10.1016/S0304-4165(01)00159-3
M3 - Article
C2 - 11479031
AN - SCOPUS:0035882235
VL - 1527
SP - 149
EP - 155
JO - Biochimica et Biophysica Acta - General Subjects
JF - Biochimica et Biophysica Acta - General Subjects
SN - 0304-4165
IS - 3
ER -