Cell age-related monovalent cations content and density changes in stored human erythrocytes

Giampaolo Minetti, Annarita Ciana, Antonella Profumo, Manuela Zappa, Cristina Vercellati, Alberto Zanella, Arduino Arduini, Augusta Brovelli

Research output: Contribution to journalArticlepeer-review


Conversion of erythrocyte membrane protein 4.1b to 4.1a occurs through a non-enzymatic deamidation reaction in most mammalian erythrocytes, with an in vivo half-life of approximately 41 days, making the 4.1a/4.1b ratio a useful index of red cell age [Inaba and Maede, Biochim. Biophys. Acta 944 (1988) 256-264]. Normal human erythrocytes distribute into subpopulations of increasing cell density and cell age when centrifuged in polyarabinogalactan density gradients. We have observed that, when erythrocytes were stored at 4°C under standard blood bank conditions, the deamidation was virtually undetectable, as cells maintained the 4.1a/4.1b ratio they displayed at the onset of storage. By measuring the 4.1a/4.1b values in subpopulations of cells of different density at various time points during storage, a modification of the normal 'cell age/cell density'] relationship was observed, as erythrocytes were affected by changes in cell volume in an age-dependent manner. This may stem from a different impact of storage on the imbalance of monovalent cations, Na+ and K+, in young and old erythrocytes, related to their different complement of cation transporters.

Original languageEnglish
Pages (from-to)149-155
Number of pages7
JournalBBA - General Subjects
Issue number3
Publication statusPublished - Aug 15 2001


  • Cell age
  • Human erythrocyte
  • Monovalent cation
  • Protein 4.1a
  • Protein 4.1b
  • Storage

ASJC Scopus subject areas

  • Biochemistry
  • Biophysics
  • Molecular Biology


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