Cell and Tissue Microarray Technologies for Protein and Nucleic Acid Expression Profiling

Marina Cardano, Giuseppe R. Diaferia, Maurizio Falavigna, Chiara C. Spinelli, Fausto Sessa, Pasquale DeBlasio, Ida Biunno

Research output: Contribution to journalArticlepeer-review

Abstract

Tissue microarray (TMA) and cell microarray (CMA) are two powerful techniques that allow for the immunophenotypical characterization of hundreds of samples simultaneously. In particular, the CMA approach is particularly useful for immunophenotyping new stem cell lines (e.g., cardiac, neural, mesenchymal) using conventional markers, as well as for testing the specificity and the efficacy of newly developed antibodies. We propose the use of a tissue arrayer not only to perform protein expression profiling by immunohistochemistry but also to carry out molecular genetics studies. In fact, starting with several tissues or cell lines, it is possible to obtain the complete signature of each sample, describing the protein, mRNA and microRNA expression, and DNA mutations, or eventually to analyze the epigenetic processes that control protein regulation. Here we show the results obtained using the Galileo CK4500 TMA platform.

Original languageEnglish
Pages (from-to)116-124
Number of pages9
JournalJournal of Histochemistry and Cytochemistry
Volume61
Issue number2
DOIs
Publication statusPublished - 2013

Keywords

  • cancer markers
  • cell type specific expression
  • IHC
  • nucleic acids
  • tissue microarray

ASJC Scopus subject areas

  • Anatomy
  • Histology
  • Medicine(all)

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