Cell-based bonding of articular cartilage: An extended study

Giuseppe M. Peretti, Victor Zaporojan, Kimberly M. Spangenberg, Mark A. Randolph, Jonathan Fellers, Lawrence J. Bonassar

Research output: Contribution to journalArticle

49 Citations (Scopus)

Abstract

This study evaluated the biomechanical characteristics of newly formed cartilaginous tissue synthesized from isolated chondrocytes and seeded onto devitalized cartilage in an extended study in vivo. Cartilage from porcine articular joints was cut into regular discs and devitalized by multiple freeze-thaw cycles. Articular chondrocytes were enzymatically isolated and incubated in suspension culture in the presence of devitalized cartilage discs for 21 days. This procedure allowed the isolated chondrocytes to adhere to the devitalized matrix surfaces. Chondrocyte-matrix constructs were assembled with fibrin glue and implanted in dorsal subcutaneous pockets in nude mice for up to 8 months. Histological evaluation and biomechanical testing were performed to quantify the integration of cartilage pieces and the mechanical properties of the constructs over time. Histological analysis indicated that chondrocytes grown on devitalized cartilage discs produced new matrix that bonded and integrated individual cartilage elements with mechanically functional tissue. Biomechanical testing demonstrated a time dependent increase in tensile strength, failure strain, failure energy, and tensile modulus to values 5-30% of normal articular cartilage by 8 months in vivo. The values recorded at 4 months were not statistically different from those collected at the latest time point, indicating that the limits of the biomechanical property values were reached after four months from implantation.

Original languageEnglish
Pages (from-to)517-524
Number of pages8
JournalJournal of Biomedical Materials Research - Part A
Volume64
Issue number3
Publication statusPublished - Mar 1 2003

Fingerprint

Cartilage
Articular Cartilage
Chondrocytes
Joints
Fibrin Tissue Adhesive
Tensile Strength
Tissue
Nude Mice
Suspensions
Glues
Swine
Testing
Tensile strength
Elastic moduli
Mechanical properties

Keywords

  • Biomechanics
  • Cartilage
  • Cartilage repair
  • Cell adhesion
  • Chondrocyte
  • Matrix bonding
  • Tissue engineering

ASJC Scopus subject areas

  • Biomedical Engineering
  • Biomaterials

Cite this

Peretti, G. M., Zaporojan, V., Spangenberg, K. M., Randolph, M. A., Fellers, J., & Bonassar, L. J. (2003). Cell-based bonding of articular cartilage: An extended study. Journal of Biomedical Materials Research - Part A, 64(3), 517-524.

Cell-based bonding of articular cartilage : An extended study. / Peretti, Giuseppe M.; Zaporojan, Victor; Spangenberg, Kimberly M.; Randolph, Mark A.; Fellers, Jonathan; Bonassar, Lawrence J.

In: Journal of Biomedical Materials Research - Part A, Vol. 64, No. 3, 01.03.2003, p. 517-524.

Research output: Contribution to journalArticle

Peretti, GM, Zaporojan, V, Spangenberg, KM, Randolph, MA, Fellers, J & Bonassar, LJ 2003, 'Cell-based bonding of articular cartilage: An extended study', Journal of Biomedical Materials Research - Part A, vol. 64, no. 3, pp. 517-524.
Peretti GM, Zaporojan V, Spangenberg KM, Randolph MA, Fellers J, Bonassar LJ. Cell-based bonding of articular cartilage: An extended study. Journal of Biomedical Materials Research - Part A. 2003 Mar 1;64(3):517-524.
Peretti, Giuseppe M. ; Zaporojan, Victor ; Spangenberg, Kimberly M. ; Randolph, Mark A. ; Fellers, Jonathan ; Bonassar, Lawrence J. / Cell-based bonding of articular cartilage : An extended study. In: Journal of Biomedical Materials Research - Part A. 2003 ; Vol. 64, No. 3. pp. 517-524.
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