Cell condensation in chondrogenic differentiation

C. Tacchetti, S. Tavella, B. Dozin, R. Quarto, G. Robino, R. Cancedda

Research output: Contribution to journalArticle

106 Citations (Scopus)

Abstract

Reduction of intercellular spaces in the areas of prospective cartilage and bone formation (precartilage condensation) precedes chondrogenesis and may represent an important step in the process of cartilage differentiation during limb skeletogenesis. We have attempted to clarify the role of the microenvironment established during cell condensation, taking advantage of a tissue culture model system that allows condensation (i.e., increased cell density due to cell aggregation) and chondrogenic differentiation (i.e., synthesis of cartilage-specific extracellular matrix proteins, such as type II collagen and acquisition of a chondrocyte morphology) of chick embryo cartilage-derived undifferentiated cells. To prevent condensation cells were grown in carboxymethylcellulose and changes in the differentiation pathway were evaluated. In another series of experiments, we have separated single cells from the aggregated cells and analyzed their differentiation properties. Morphological analyses and the evaluation of type II collagen expression, at both the protein and the mRNA level, show that a reduced rate of cell clustering and cell to cell contact parallels a reduction of cell recruitment into the differentiation program. On the basis of our results, we suggest that the following cascade of events regulates the early stages of chondrocyte differentiation: (a) the acquisition of the ability to establish cell to cell contacts, (b) the formation of a permissive environment capable of activating the differentiation program, and (c) the expression of differentiation markers.

Original languageEnglish
Pages (from-to)26-33
Number of pages8
JournalExperimental Cell Research
Volume200
Issue number1
DOIs
Publication statusPublished - 1992

Fingerprint

Cartilage
Collagen Type II
Chondrocytes
Chondrogenesis
Cell Aggregation
Carboxymethylcellulose Sodium
Extracellular Matrix Proteins
Differentiation Antigens
Extracellular Space
Chick Embryo
Osteogenesis
Cluster Analysis
Extremities
Cell Count
Messenger RNA
Proteins

ASJC Scopus subject areas

  • Cell Biology

Cite this

Cell condensation in chondrogenic differentiation. / Tacchetti, C.; Tavella, S.; Dozin, B.; Quarto, R.; Robino, G.; Cancedda, R.

In: Experimental Cell Research, Vol. 200, No. 1, 1992, p. 26-33.

Research output: Contribution to journalArticle

Tacchetti, C. ; Tavella, S. ; Dozin, B. ; Quarto, R. ; Robino, G. ; Cancedda, R. / Cell condensation in chondrogenic differentiation. In: Experimental Cell Research. 1992 ; Vol. 200, No. 1. pp. 26-33.
@article{15c042d081da496a8e363a62bc79e83b,
title = "Cell condensation in chondrogenic differentiation",
abstract = "Reduction of intercellular spaces in the areas of prospective cartilage and bone formation (precartilage condensation) precedes chondrogenesis and may represent an important step in the process of cartilage differentiation during limb skeletogenesis. We have attempted to clarify the role of the microenvironment established during cell condensation, taking advantage of a tissue culture model system that allows condensation (i.e., increased cell density due to cell aggregation) and chondrogenic differentiation (i.e., synthesis of cartilage-specific extracellular matrix proteins, such as type II collagen and acquisition of a chondrocyte morphology) of chick embryo cartilage-derived undifferentiated cells. To prevent condensation cells were grown in carboxymethylcellulose and changes in the differentiation pathway were evaluated. In another series of experiments, we have separated single cells from the aggregated cells and analyzed their differentiation properties. Morphological analyses and the evaluation of type II collagen expression, at both the protein and the mRNA level, show that a reduced rate of cell clustering and cell to cell contact parallels a reduction of cell recruitment into the differentiation program. On the basis of our results, we suggest that the following cascade of events regulates the early stages of chondrocyte differentiation: (a) the acquisition of the ability to establish cell to cell contacts, (b) the formation of a permissive environment capable of activating the differentiation program, and (c) the expression of differentiation markers.",
author = "C. Tacchetti and S. Tavella and B. Dozin and R. Quarto and G. Robino and R. Cancedda",
year = "1992",
doi = "10.1016/S0014-4827(05)80067-9",
language = "English",
volume = "200",
pages = "26--33",
journal = "Experimental Cell Research",
issn = "0014-4827",
publisher = "Academic Press Inc.",
number = "1",

}

TY - JOUR

T1 - Cell condensation in chondrogenic differentiation

AU - Tacchetti, C.

AU - Tavella, S.

AU - Dozin, B.

AU - Quarto, R.

AU - Robino, G.

AU - Cancedda, R.

PY - 1992

Y1 - 1992

N2 - Reduction of intercellular spaces in the areas of prospective cartilage and bone formation (precartilage condensation) precedes chondrogenesis and may represent an important step in the process of cartilage differentiation during limb skeletogenesis. We have attempted to clarify the role of the microenvironment established during cell condensation, taking advantage of a tissue culture model system that allows condensation (i.e., increased cell density due to cell aggregation) and chondrogenic differentiation (i.e., synthesis of cartilage-specific extracellular matrix proteins, such as type II collagen and acquisition of a chondrocyte morphology) of chick embryo cartilage-derived undifferentiated cells. To prevent condensation cells were grown in carboxymethylcellulose and changes in the differentiation pathway were evaluated. In another series of experiments, we have separated single cells from the aggregated cells and analyzed their differentiation properties. Morphological analyses and the evaluation of type II collagen expression, at both the protein and the mRNA level, show that a reduced rate of cell clustering and cell to cell contact parallels a reduction of cell recruitment into the differentiation program. On the basis of our results, we suggest that the following cascade of events regulates the early stages of chondrocyte differentiation: (a) the acquisition of the ability to establish cell to cell contacts, (b) the formation of a permissive environment capable of activating the differentiation program, and (c) the expression of differentiation markers.

AB - Reduction of intercellular spaces in the areas of prospective cartilage and bone formation (precartilage condensation) precedes chondrogenesis and may represent an important step in the process of cartilage differentiation during limb skeletogenesis. We have attempted to clarify the role of the microenvironment established during cell condensation, taking advantage of a tissue culture model system that allows condensation (i.e., increased cell density due to cell aggregation) and chondrogenic differentiation (i.e., synthesis of cartilage-specific extracellular matrix proteins, such as type II collagen and acquisition of a chondrocyte morphology) of chick embryo cartilage-derived undifferentiated cells. To prevent condensation cells were grown in carboxymethylcellulose and changes in the differentiation pathway were evaluated. In another series of experiments, we have separated single cells from the aggregated cells and analyzed their differentiation properties. Morphological analyses and the evaluation of type II collagen expression, at both the protein and the mRNA level, show that a reduced rate of cell clustering and cell to cell contact parallels a reduction of cell recruitment into the differentiation program. On the basis of our results, we suggest that the following cascade of events regulates the early stages of chondrocyte differentiation: (a) the acquisition of the ability to establish cell to cell contacts, (b) the formation of a permissive environment capable of activating the differentiation program, and (c) the expression of differentiation markers.

UR - http://www.scopus.com/inward/record.url?scp=0026711097&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=0026711097&partnerID=8YFLogxK

U2 - 10.1016/S0014-4827(05)80067-9

DO - 10.1016/S0014-4827(05)80067-9

M3 - Article

VL - 200

SP - 26

EP - 33

JO - Experimental Cell Research

JF - Experimental Cell Research

SN - 0014-4827

IS - 1

ER -