Cell-cycle dependent alternative splicing of the tenascin primary transcript

Laura Borsi, Enrica Balza, Patrizia Castellani, Barbara Carnemolla, Marco Ponassi, Germano Querzé, Luciano Zardi

Research output: Contribution to journalArticle

Abstract

Functionally different tenascin (TN) isoforms may be generated by alternative splicing of the TN primary transcript. In fact, it has been demonstrated that only the larger TN isoform containing the alternatively spliced region induces loss of focal adhesion in cultured cells and seems able to facilitate cell migration. Recent studies have shown that the higher molecular mass TN isoform is a marker of stromal cell proliferation in hyperplastic and neoplastic breast tissues. This finding prompted us to study the pattern of TN alternative splicing in proliferating and non-proliferating cultured fibroblasts. Here, we show that the mitogenic stimulation of fibroblasts with serum or cytokines leads to an early and striking modification in the steady-state levels of the two major TN mRNAs. We also show that de novo protein synthesis is not necessary for this modification, indicating that it is a "primary response" event. Similarly, mitogenic stimulation induces changes both in synthesis and accumulation of the different TN isoforms.

Original languageEnglish
Pages (from-to)307-317
Number of pages11
JournalCell Communication and Adhesion
Volume1
Issue number4
DOIs
Publication statusPublished - 1994

ASJC Scopus subject areas

  • Cell Biology
  • Clinical Biochemistry

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