Cell cycle-dependent induction of autophagy, mitophagy and reticulophagy

Ezgi Tasdemir, M. Chiara Maiuri, Nicolas Tajeddine, Ilio Vitale, Alfredo Criollo, José Miguel Vicencio, John A. Hickman, Olivier Geneste, Guido Kroemer

Research output: Contribution to journalArticle

Abstract

When added to cells, a variety of autophagy inducers that operate through distinct mechanisms and target different organelles for autophagic destruction (mitochondria in mitophagy, endoplasmic reticulum in reticulophagy) rarely induce autophagic vacuolization in more than 50% or the cells. Here we show that this heterogeneity may be explained by cell cycle-specific effects. The BH3 mimetic ABT737, lithium, rapamycin, tunicamycin or nutrient depletion stereotypically induce autophagy preferentially in the G1 and S phases of the cell cycle, as determined by simultaneous monitoring of cell cycle markers and the cytoplasmic aggregation of GFP-LC3 in autophagic vacuoles. These results point to a hitherto neglected crosstalk between autophagic vacuolization and cell cycle regulation.

Original languageEnglish
Pages (from-to)2263-2267
Number of pages5
JournalCell Cycle
Volume6
Issue number18
Publication statusPublished - Sep 15 2007

Keywords

  • Autophagy
  • Cell cycle
  • Endoplasmic reticulum
  • Mitochondria

ASJC Scopus subject areas

  • Cell Biology
  • Biochemistry
  • Molecular Biology

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  • Cite this

    Tasdemir, E., Maiuri, M. C., Tajeddine, N., Vitale, I., Criollo, A., Vicencio, J. M., Hickman, J. A., Geneste, O., & Kroemer, G. (2007). Cell cycle-dependent induction of autophagy, mitophagy and reticulophagy. Cell Cycle, 6(18), 2263-2267.