Cell cycle synchronization of FRTL5 cells. A physiological model system

A. Degrassi, M. C G Monaco, G. Lisignoli, O. Belvedere, S. Toneguzzi, W. Malangone, M. L. Bonora, A. Piacentini, S. Lavaroni, M. Scarbolo, F. S. Ambesi-Impiombato, A. Facchini

Research output: Contribution to journalArticle

Abstract

We describe a 'physiological' cell cycle synchronization model system. FRTL5 cells, TSH-dependent for proliferation, were starved from TSH. The cell cycle phases and the expression of markers associated to different cycle phases were evaluated. TSH starvation blocks proliferation without provoking death and induces virtually all the cells to accumulate in G0/G1 phase. TSH readdition allows 30% of these cells to enter the S phase. DNA topoisomerase II 170-kDa isoform is not expressed in G0/G1 synchronized cells while it is expressed in logarithmic growing cells. The 180-kDa isoform is not expressed in G0/G1 synchronized cells while it is expressed in 20% of logarithmic growing cells regardless of the cycle phase. c-myc mRNA is not expressed in G0/G1 synchronized cells while it is detectable upon TSH readdition. This system provides a tool for the analysis of events associated with the G0/G1 phase and the transition from G0/G1 to S phase.

Original languageEnglish
Pages (from-to)527-532
Number of pages6
JournalJournal of Experimental and Clinical Cancer Research
Volume17
Issue number4
Publication statusPublished - Dec 1998

Keywords

  • C-myc
  • Cell cycle synchronization
  • DNA topoisomerase II
  • FRTL5 cells

ASJC Scopus subject areas

  • Cancer Research
  • Oncology

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    Degrassi, A., Monaco, M. C. G., Lisignoli, G., Belvedere, O., Toneguzzi, S., Malangone, W., Bonora, M. L., Piacentini, A., Lavaroni, S., Scarbolo, M., Ambesi-Impiombato, F. S., & Facchini, A. (1998). Cell cycle synchronization of FRTL5 cells. A physiological model system. Journal of Experimental and Clinical Cancer Research, 17(4), 527-532.