Cell death in the olfactory epithelium

Lorenzo Magrassi, Pasquale P C Graziadei

Research output: Contribution to journalArticle

Abstract

In the nervous system of vertebrates the olfactory epithelium presents unique cytological characteristics. In the olfactory mucosa, olfactory neurons die and are replaced from undifferentiated neuroblasts over the entire life span of the animal. It remains unclear whether these neurons die as a result of a direct insult from the environment or in fulfillment of a physiological program of cell death. We have studied the distribution and the characteristics of cell death in the olfactory epithelium of normal, adult rats. The olfactory epithelium contains pycnotic bodies resembling those described for thymocytes undergoing terminal apoptotic changes. These appear at all levels in the epithelium, under both light and electron microscopes and can also be demonstrated after vital staining with acridine orange. Chromatin condensation into large blocks, often located at the nuclear periphery, is a morphological hallmark of the nuclei of mature olfactory neurons, which also present an increase in electron density of the cytoplasm. After non-radioactive in situ labeling of fragmented DNA, the nuclei of olfactory neurons are positive. Under the same reaction conditions (mild protease digestion), most of the nuclei of the supporting and basal cells are negative. In vivo incorporation of 5-bromouridine, a marker of RNA synthesis, is also lower in olfactory neurons than in basal and supporting cells. These findings suggest that olfactory neurons are committed very early to physiological cell death.

Original languageEnglish
Pages (from-to)77-87
Number of pages11
JournalAnatomy and Embryology
Volume192
Issue number1
DOIs
Publication statusPublished - Jul 1995

Fingerprint

Olfactory Mucosa
Cell Death
Neurons
Electrons
Acridine Orange
Thymocytes
Basal Ganglia
Nervous System
Chromatin
Vertebrates
Digestion
Cytoplasm
Peptide Hydrolases
Epithelium
RNA
Staining and Labeling
Light
DNA

Keywords

  • Apoptosis
  • Cell death
  • Fragmented DNA
  • Olfactory epithelium
  • RNA synthesis

ASJC Scopus subject areas

  • Embryology
  • Anatomy
  • Developmental Biology
  • Cell Biology

Cite this

Cell death in the olfactory epithelium. / Magrassi, Lorenzo; Graziadei, Pasquale P C.

In: Anatomy and Embryology, Vol. 192, No. 1, 07.1995, p. 77-87.

Research output: Contribution to journalArticle

Magrassi, Lorenzo ; Graziadei, Pasquale P C. / Cell death in the olfactory epithelium. In: Anatomy and Embryology. 1995 ; Vol. 192, No. 1. pp. 77-87.
@article{f8b41f1cea7d4ec08e8b7aba5e5d6661,
title = "Cell death in the olfactory epithelium",
abstract = "In the nervous system of vertebrates the olfactory epithelium presents unique cytological characteristics. In the olfactory mucosa, olfactory neurons die and are replaced from undifferentiated neuroblasts over the entire life span of the animal. It remains unclear whether these neurons die as a result of a direct insult from the environment or in fulfillment of a physiological program of cell death. We have studied the distribution and the characteristics of cell death in the olfactory epithelium of normal, adult rats. The olfactory epithelium contains pycnotic bodies resembling those described for thymocytes undergoing terminal apoptotic changes. These appear at all levels in the epithelium, under both light and electron microscopes and can also be demonstrated after vital staining with acridine orange. Chromatin condensation into large blocks, often located at the nuclear periphery, is a morphological hallmark of the nuclei of mature olfactory neurons, which also present an increase in electron density of the cytoplasm. After non-radioactive in situ labeling of fragmented DNA, the nuclei of olfactory neurons are positive. Under the same reaction conditions (mild protease digestion), most of the nuclei of the supporting and basal cells are negative. In vivo incorporation of 5-bromouridine, a marker of RNA synthesis, is also lower in olfactory neurons than in basal and supporting cells. These findings suggest that olfactory neurons are committed very early to physiological cell death.",
keywords = "Apoptosis, Cell death, Fragmented DNA, Olfactory epithelium, RNA synthesis",
author = "Lorenzo Magrassi and Graziadei, {Pasquale P C}",
year = "1995",
month = "7",
doi = "10.1007/BF00186993",
language = "English",
volume = "192",
pages = "77--87",
journal = "Anatomische Hefte",
issn = "0177-5154",
publisher = "Springer Verlag",
number = "1",

}

TY - JOUR

T1 - Cell death in the olfactory epithelium

AU - Magrassi, Lorenzo

AU - Graziadei, Pasquale P C

PY - 1995/7

Y1 - 1995/7

N2 - In the nervous system of vertebrates the olfactory epithelium presents unique cytological characteristics. In the olfactory mucosa, olfactory neurons die and are replaced from undifferentiated neuroblasts over the entire life span of the animal. It remains unclear whether these neurons die as a result of a direct insult from the environment or in fulfillment of a physiological program of cell death. We have studied the distribution and the characteristics of cell death in the olfactory epithelium of normal, adult rats. The olfactory epithelium contains pycnotic bodies resembling those described for thymocytes undergoing terminal apoptotic changes. These appear at all levels in the epithelium, under both light and electron microscopes and can also be demonstrated after vital staining with acridine orange. Chromatin condensation into large blocks, often located at the nuclear periphery, is a morphological hallmark of the nuclei of mature olfactory neurons, which also present an increase in electron density of the cytoplasm. After non-radioactive in situ labeling of fragmented DNA, the nuclei of olfactory neurons are positive. Under the same reaction conditions (mild protease digestion), most of the nuclei of the supporting and basal cells are negative. In vivo incorporation of 5-bromouridine, a marker of RNA synthesis, is also lower in olfactory neurons than in basal and supporting cells. These findings suggest that olfactory neurons are committed very early to physiological cell death.

AB - In the nervous system of vertebrates the olfactory epithelium presents unique cytological characteristics. In the olfactory mucosa, olfactory neurons die and are replaced from undifferentiated neuroblasts over the entire life span of the animal. It remains unclear whether these neurons die as a result of a direct insult from the environment or in fulfillment of a physiological program of cell death. We have studied the distribution and the characteristics of cell death in the olfactory epithelium of normal, adult rats. The olfactory epithelium contains pycnotic bodies resembling those described for thymocytes undergoing terminal apoptotic changes. These appear at all levels in the epithelium, under both light and electron microscopes and can also be demonstrated after vital staining with acridine orange. Chromatin condensation into large blocks, often located at the nuclear periphery, is a morphological hallmark of the nuclei of mature olfactory neurons, which also present an increase in electron density of the cytoplasm. After non-radioactive in situ labeling of fragmented DNA, the nuclei of olfactory neurons are positive. Under the same reaction conditions (mild protease digestion), most of the nuclei of the supporting and basal cells are negative. In vivo incorporation of 5-bromouridine, a marker of RNA synthesis, is also lower in olfactory neurons than in basal and supporting cells. These findings suggest that olfactory neurons are committed very early to physiological cell death.

KW - Apoptosis

KW - Cell death

KW - Fragmented DNA

KW - Olfactory epithelium

KW - RNA synthesis

UR - http://www.scopus.com/inward/record.url?scp=0029011828&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=0029011828&partnerID=8YFLogxK

U2 - 10.1007/BF00186993

DO - 10.1007/BF00186993

M3 - Article

C2 - 7486003

AN - SCOPUS:0029011828

VL - 192

SP - 77

EP - 87

JO - Anatomische Hefte

JF - Anatomische Hefte

SN - 0177-5154

IS - 1

ER -