Abstract
The death of target cells by cytotoxic effector cells is a relevant biological phenomenon, where cells are activated and a very quick apoptotic program occurs. In order to test the hypothesis that the nuclear enzyme poly(ADP-ribose)polymerase (PADPRP) plays a role in such a process, a variety of PADPRP inhibitors such as 3-aminobenzamide, nicotinamide, 4-aminobenzamide and luminol were used. All of them were able to strongly inhibit K562 target cell killing by human effector natural killer cells (NK) in a 4hr 51Cr release assay. PADPRP inhibitors were much less effective in protecting target cells when lymphokine activated killer cells (LAK) were used as effectors. These substances were active only when both target and effector cells were mixed, being ineffective on target or effector cells alone. On the whole, these data indicate that PADPRP is involved in the death of target cells. Moreover, the different sensitivity of NK and LAK activities to PADPRP inhibitors suggests that the molecular mechanisms underlying these two types of cytotoxicity are at least partially different.
| Original language | English |
|---|---|
| Pages (from-to) | 525-530 |
| Number of pages | 6 |
| Journal | Biochemical and Biophysical Research Communications |
| Volume | 199 |
| Issue number | 2 |
| DOIs | |
| Publication status | Published - 1994 |
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ASJC Scopus subject areas
- Biochemistry
- Biophysics
- Molecular Biology
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Cell death protection by 3-aminobenzamide and other poly(ADP-ribose)polymerase inhibitors : Different effects on human natural killer and lymphokine activated killer cell activities. / Monti, D.; Cossarizza, A.; Salvioli, S.; Franceschi, C.; Rainaldi, G.; Straface, E.; Rivabene, R.; Malorni, W.
In: Biochemical and Biophysical Research Communications, Vol. 199, No. 2, 1994, p. 525-530.Research output: Contribution to journal › Article
}
TY - JOUR
T1 - Cell death protection by 3-aminobenzamide and other poly(ADP-ribose)polymerase inhibitors
T2 - Different effects on human natural killer and lymphokine activated killer cell activities
AU - Monti, D.
AU - Cossarizza, A.
AU - Salvioli, S.
AU - Franceschi, C.
AU - Rainaldi, G.
AU - Straface, E.
AU - Rivabene, R.
AU - Malorni, W.
PY - 1994
Y1 - 1994
N2 - The death of target cells by cytotoxic effector cells is a relevant biological phenomenon, where cells are activated and a very quick apoptotic program occurs. In order to test the hypothesis that the nuclear enzyme poly(ADP-ribose)polymerase (PADPRP) plays a role in such a process, a variety of PADPRP inhibitors such as 3-aminobenzamide, nicotinamide, 4-aminobenzamide and luminol were used. All of them were able to strongly inhibit K562 target cell killing by human effector natural killer cells (NK) in a 4hr 51Cr release assay. PADPRP inhibitors were much less effective in protecting target cells when lymphokine activated killer cells (LAK) were used as effectors. These substances were active only when both target and effector cells were mixed, being ineffective on target or effector cells alone. On the whole, these data indicate that PADPRP is involved in the death of target cells. Moreover, the different sensitivity of NK and LAK activities to PADPRP inhibitors suggests that the molecular mechanisms underlying these two types of cytotoxicity are at least partially different.
AB - The death of target cells by cytotoxic effector cells is a relevant biological phenomenon, where cells are activated and a very quick apoptotic program occurs. In order to test the hypothesis that the nuclear enzyme poly(ADP-ribose)polymerase (PADPRP) plays a role in such a process, a variety of PADPRP inhibitors such as 3-aminobenzamide, nicotinamide, 4-aminobenzamide and luminol were used. All of them were able to strongly inhibit K562 target cell killing by human effector natural killer cells (NK) in a 4hr 51Cr release assay. PADPRP inhibitors were much less effective in protecting target cells when lymphokine activated killer cells (LAK) were used as effectors. These substances were active only when both target and effector cells were mixed, being ineffective on target or effector cells alone. On the whole, these data indicate that PADPRP is involved in the death of target cells. Moreover, the different sensitivity of NK and LAK activities to PADPRP inhibitors suggests that the molecular mechanisms underlying these two types of cytotoxicity are at least partially different.
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UR - http://www.scopus.com/inward/citedby.url?scp=0028214198&partnerID=8YFLogxK
U2 - 10.1006/bbrc.1994.1260
DO - 10.1006/bbrc.1994.1260
M3 - Article
C2 - 8135793
AN - SCOPUS:0028214198
VL - 199
SP - 525
EP - 530
JO - Biochemical and Biophysical Research Communications
JF - Biochemical and Biophysical Research Communications
SN - 0006-291X
IS - 2
ER -