Cell density modulates SHC3 expression and survival of human glioblastoma cells through Fak activation

Alberto Azzalin, Elena Moretti, Eloisa Arbustini, Lorenzo Magrassi

Research output: Contribution to journalArticlepeer-review

Abstract

Shc3 protein levels are high in human glioblastoma but they decrease in vitro. We found that SHC3 mRNA and protein increased when glioblastoma cells grew as multicellular tumor spheroid (MTS). Shc3 expression was also induced in adherent cultures by increasing cell density. Among the Shc family members, only Shc2 and Shc3 increased with cell density. Shc3 and focal adhesion kinase (Fak) interact as shown by co-immunoprecipitation. Inhibition of Fak activation reduced Shc3 increase and MTS formation and changed Shc3 phosphorylation pattern. Our results suggest that in gliomas cell density modulates Shc3 protein levels and its activity, at least in part, through Fak activation.

Original languageEnglish
Pages (from-to)245-256
Number of pages12
JournalJournal of Neuro-Oncology
Volume120
Issue number2
DOIs
Publication statusPublished - Oct 22 2014

Keywords

  • Cell cell contacts
  • Fak
  • Glioblastoma
  • Multicellular tumor spheroid
  • Shc3

ASJC Scopus subject areas

  • Clinical Neurology
  • Cancer Research
  • Oncology
  • Neurology
  • Medicine(all)

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