TY - JOUR
T1 - Cell discohesion and multifocality of carcinoma in situ of the bladder
T2 - New insight from the adhesion molecule profile (e-Cadherin, Ep-CAM, and MUC1)
AU - Patriarca, Carlo
AU - Colombo, Piergiuseppe
AU - Pio Taronna, Angelo
AU - Wesseling, Jelle
AU - Franchi, Giada
AU - Guddo, Francesca
AU - Naspro, Richard
AU - MacChi, Roberto Maria
AU - Giunta, Paolo
AU - Di Pasquale, Marcello
AU - Parente, Michele
AU - Arizzi, Carmelo
AU - Roncalli, Massimo
AU - Campo, Biagio
PY - 2009/4
Y1 - 2009/4
N2 - Urothelial cell carcinoma in situ (CIS) of the bladder is a superficially diffusive and highly discohesive disease. The authors analyzed the expression of some adhesion molecules (e-cadherin and Ep-CAM) and MUC1 in 32 unifocal and multifocal bladder urothelial cell CIS in an attempt to clarify this discohesion. E-cadherin was strongly expressed, in more than 75% of the cases. The presence of methylation of the CDH1 e-cadherin promoter gene was also investigated, but methylation was found in only one case. Ep-CAM was present in all the cases with a heterogeneous staining pattern. Similarly, MUC1/episialin was variously present in 94% of the cases without a polarized staining pattern and was expressed more strongly in cases with multifocal disease. Because loss of MUC1 polarization leads to interference with cell-cell adhesion mechanisms mediated by cadherins, these findings help explain why bladder urothelial cell CIS often shows a discohesive morphology and multifocality despite a strongly expressed adhesion molecule profile. Finally, Ep-CAM expression might provide some support for future target therapy trials.
AB - Urothelial cell carcinoma in situ (CIS) of the bladder is a superficially diffusive and highly discohesive disease. The authors analyzed the expression of some adhesion molecules (e-cadherin and Ep-CAM) and MUC1 in 32 unifocal and multifocal bladder urothelial cell CIS in an attempt to clarify this discohesion. E-cadherin was strongly expressed, in more than 75% of the cases. The presence of methylation of the CDH1 e-cadherin promoter gene was also investigated, but methylation was found in only one case. Ep-CAM was present in all the cases with a heterogeneous staining pattern. Similarly, MUC1/episialin was variously present in 94% of the cases without a polarized staining pattern and was expressed more strongly in cases with multifocal disease. Because loss of MUC1 polarization leads to interference with cell-cell adhesion mechanisms mediated by cadherins, these findings help explain why bladder urothelial cell CIS often shows a discohesive morphology and multifocality despite a strongly expressed adhesion molecule profile. Finally, Ep-CAM expression might provide some support for future target therapy trials.
KW - Bladder
KW - Carcinoma in situ (CIS)
KW - Discohesion
KW - E-cadherin
KW - MUC1
KW - Urothelial cell carcinoma
UR - http://www.scopus.com/inward/record.url?scp=62849126628&partnerID=8YFLogxK
UR - http://www.scopus.com/inward/citedby.url?scp=62849126628&partnerID=8YFLogxK
U2 - 10.1177/1066896908326918
DO - 10.1177/1066896908326918
M3 - Article
C2 - 19019843
AN - SCOPUS:62849126628
VL - 17
SP - 99
EP - 106
JO - International Journal of Surgical Pathology
JF - International Journal of Surgical Pathology
SN - 1066-8969
IS - 2
ER -