Cell dynamics in the correct control of bone metabolism using natural treatments

Monica Mattioli-Belmonte, K. Kyriakidou, G. Lucarini, O. Gorrieri, G. Giavaresi, M. Fini, R. Giardino, S. Amati, G. Suffritti, G. Biagini

Research output: Contribution to journalArticlepeer-review


The present study was undertaken in order to assess the efficacy of a commercial product containing calcium and silicon (Osteosil-Calcium®) on cell metabolism. MG-63 osteblast-like cells were cultured in the presence of three different drug concentrations (10, 5 and 2.5 μg/mL). Either serum-free culture and standard culture with serum were investigated. Morpho-functional tests (MTT and ALP), scanning electron microscopy (SEM), microanalysis (EDAX) and time-lapse video microscopy were performed. Cell actin cytoskeletal modification with fluorescence phalloidin staining was also tested. Our data show the in vitro functional efficacy of Osteosil-Calcium® on MG63 cell viability and ALP production. This study demonstrates its positive effect on the metabolism of the single cell and suggests wider uses of this drug in health protection and or in Regenerative Medicine therapies which are currently applied to the elderly.

Original languageEnglish
Pages (from-to)1259-1271
Number of pages13
JournalInternational Journal of Artificial Organs
Issue number12
Publication statusPublished - Dec 2005


  • Alkaline phosphatase
  • Ca
  • Cell behavior
  • EDAX
  • MG63 cells
  • Time lapse video-microscopy

ASJC Scopus subject areas

  • Biophysics


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