TY - JOUR
T1 - Cell dynamics in the correct control of bone metabolism using natural treatments
AU - Mattioli-Belmonte, Monica
AU - Kyriakidou, K.
AU - Lucarini, G.
AU - Gorrieri, O.
AU - Giavaresi, G.
AU - Fini, M.
AU - Giardino, R.
AU - Amati, S.
AU - Suffritti, G.
AU - Biagini, G.
PY - 2005/12
Y1 - 2005/12
N2 - The present study was undertaken in order to assess the efficacy of a commercial product containing calcium and silicon (Osteosil-Calcium®) on cell metabolism. MG-63 osteblast-like cells were cultured in the presence of three different drug concentrations (10, 5 and 2.5 μg/mL). Either serum-free culture and standard culture with serum were investigated. Morpho-functional tests (MTT and ALP), scanning electron microscopy (SEM), microanalysis (EDAX) and time-lapse video microscopy were performed. Cell actin cytoskeletal modification with fluorescence phalloidin staining was also tested. Our data show the in vitro functional efficacy of Osteosil-Calcium® on MG63 cell viability and ALP production. This study demonstrates its positive effect on the metabolism of the single cell and suggests wider uses of this drug in health protection and or in Regenerative Medicine therapies which are currently applied to the elderly.
AB - The present study was undertaken in order to assess the efficacy of a commercial product containing calcium and silicon (Osteosil-Calcium®) on cell metabolism. MG-63 osteblast-like cells were cultured in the presence of three different drug concentrations (10, 5 and 2.5 μg/mL). Either serum-free culture and standard culture with serum were investigated. Morpho-functional tests (MTT and ALP), scanning electron microscopy (SEM), microanalysis (EDAX) and time-lapse video microscopy were performed. Cell actin cytoskeletal modification with fluorescence phalloidin staining was also tested. Our data show the in vitro functional efficacy of Osteosil-Calcium® on MG63 cell viability and ALP production. This study demonstrates its positive effect on the metabolism of the single cell and suggests wider uses of this drug in health protection and or in Regenerative Medicine therapies which are currently applied to the elderly.
KW - Alkaline phosphatase
KW - Ca
KW - Cell behavior
KW - EDAX
KW - MG63 cells
KW - Time lapse video-microscopy
UR - http://www.scopus.com/inward/record.url?scp=31644440544&partnerID=8YFLogxK
UR - http://www.scopus.com/inward/citedby.url?scp=31644440544&partnerID=8YFLogxK
M3 - Article
C2 - 16404703
AN - SCOPUS:31644440544
VL - 28
SP - 1259
EP - 1271
JO - International Journal of Artificial Organs
JF - International Journal of Artificial Organs
SN - 0391-3988
IS - 12
ER -