Cell fusion in tumor progression: The isolation of cell fusion products by physical methods

Filippo Pedrazzoli, Iraklis Chrysantzas, Luca Dezzani, Vittorio Rosti, Massimo Vincitorio, Giammaria Sitar

Research output: Contribution to journalArticle

Abstract

Background: Cell fusion induced by polyethylene glycol (PEG) is an efficient but poorly controlled procedure for obtaining somatic cell hybrids used in gene mapping, monoclonal antibody production, and tumour immunotherapy. Genetic selection techniques and fluorescent cell sorting are usually employed to isolate cell fusion products, but both procedures have several drawbacks.Results: Here we describe a simple improvement in PEG-mediated cell fusion that was obtained by modifying the standard single-step procedure. We found that the use of two PEG undertreatments obtains a better yield of cell fusion products than the standard method, and most of these products are bi- or trinucleated polykaryocytes. Fusion rate was quantified using fluorescent cell staining microscopy. We used this improved cell fusion and cell isolation method to compare giant cells obtained in vitro and giant cells obtained in vivo from patients with Hodgkin's disease and erythroleukemia.Conclusions: In the present study we show how to improve PEG-mediated cell fusion and that cell separation by velocity sedimentation offers a simple alternative for the efficient purification of cell fusion products and to investigate giant cell formation in tumor development.

Original languageEnglish
Article number32
JournalCancer Cell International
Volume11
DOIs
Publication statusPublished - Sep 20 2011

ASJC Scopus subject areas

  • Cancer Research
  • Oncology
  • Genetics

Fingerprint Dive into the research topics of 'Cell fusion in tumor progression: The isolation of cell fusion products by physical methods'. Together they form a unique fingerprint.

  • Cite this