Cell manipulation in autologous chondrocyte implantation: from research to cleanroom.

Livia Roseti, Marta Serra, Domenico Tigani, Irene Brognara, Annamaria Lopriore, Alessandra Bassi, Pier Maria Fornasari

Research output: Contribution to journalArticle

Abstract

In the field of orthopaedics, autologous chondrocyte implantation is a technique currently used for the regeneration of damaged articular cartilage. There is evidence of the neo-formation of tissue displaying characteristics similar to hyaline cartilage. In vitro chondrocyte manipulation is a crucial phase of this therapeutic treatment consisting of different steps: cell isolation from a cartilage biopsy, expansion in monolayer culture and growth onto a three-dimensional biomaterial to implant in the damaged area. To minimise the risk of in vitro cell contamination, the manipulation must be performed in a controlled environment such as a cleanroom. Moreover, the choice of reagents and raw material suitable for clinical use in humans and the translation of research protocols into standardised production processes are important. In this study we describe the preliminary results obtained by the development of chondrocyte manipulation protocols (isolation and monolayer expansion) in cleanrooms for the application of autologous implantation.

Original languageEnglish
Pages (from-to)147-151
Number of pages5
JournalLa Chirurgia degli organi di movimento
Volume91
Issue number3
DOIs
Publication statusPublished - Apr 2008

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Chondrocytes
Research
Hyaline Cartilage
Controlled Environment
Cell Separation
Articular Cartilage
Biocompatible Materials
Cartilage
Orthopedics
Regeneration
Biopsy
Growth
In Vitro Techniques
Therapeutics

ASJC Scopus subject areas

  • Medicine(all)

Cite this

Cell manipulation in autologous chondrocyte implantation : from research to cleanroom. / Roseti, Livia; Serra, Marta; Tigani, Domenico; Brognara, Irene; Lopriore, Annamaria; Bassi, Alessandra; Fornasari, Pier Maria.

In: La Chirurgia degli organi di movimento, Vol. 91, No. 3, 04.2008, p. 147-151.

Research output: Contribution to journalArticle

Roseti, Livia ; Serra, Marta ; Tigani, Domenico ; Brognara, Irene ; Lopriore, Annamaria ; Bassi, Alessandra ; Fornasari, Pier Maria. / Cell manipulation in autologous chondrocyte implantation : from research to cleanroom. In: La Chirurgia degli organi di movimento. 2008 ; Vol. 91, No. 3. pp. 147-151.
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