Cell-mediated and antibody responses to bordetella pertussis antigens in children vaccinated with acellular or whole-cell pertussis vaccines

Antonio Cossone, Clara M. Ausiello, Francesca Urbani, Roberta Lande, Marina Giuliano, Andrea La Sala, Annamaria Piscitelli, Stefania Salmaso

Research output: Contribution to journalArticle

66 Citations (Scopus)

Abstract

Objective: To examine induction and persistence of cell-mediated immunity (CMI) and antibody responses to Bordetella pertussis antigens in infants receiving antipertussis vaccines. Design and Setting: A randomized, blinded study of 142 children receiving acellular pertussis vaccines combined with diphtheria-tetanus toxoids (DTaP) (DTaP manufactured by SmithKline Beecham [DTaP-SB], Rixensart, Belgium, and DTaP manufactured by Chiron Biocin [DTaP-CB], Siena, Italy), or a whole-cell pertussis vaccine (DTwP) (Connaught Laboratories Inc, Swiftwater, Pa), or a diphtheria-tetanus (DT) (Chiron Biocine) only vaccine. Three doses of each vaccine were given at 2, 4, and 6 months of age, and CMI and antibody responses were evaluated before and at 1 and 14 months after vaccination. Methods and Main Outcome Measures: Cell- mediated immunity was assessed by proliferation of peripheral blood mononuclear cells stimulated in vitro by B pertussis antigens (pertussis toxin, filamentous hemagglutinin, and pertactin). Antibody titers against pertussis toxin, filamentous hemagglutinin, and pertactin were determined by a standardized enzyme-linked immunosorbent assay. Results: A CMI-positive response to at least 1 B pertussis antigen at 1 or both postvaccination assays was detected in 46%, 55%, and 83% of DTwP, DTaP-SB, and DTaP-CB vaccine recipients, respectively. Frequency of CMI response to individual antigens ranged from less than 4.9% against pertussis toxin in DTwP recipients to 52% against pertactin in DTaP-CB recipients. The postvaccination responses measured at 14 months equalled, or had increased frequency or intensity, that of the 1-month postvaccination responses. Elevated antibody titers against the 3 antigens were present in all DTaP recipients 1 month after vaccination and were higher in CMI-positive children than in CMl-negative children. They fell, however, to low, if not negligible, levels 14 months after vaccination. Conclusions: Acellular pertussis vaccines were better inducers of CMI response than the whole-cell vaccine, particularly against pertussis toxin. Once acquired, CMI persisted, in contrast with the rapid antibody decline. Thus, CMI responses could be a useful adjunct to serology in the evaluation of pertussis vaccine immunogenicity and a better correlate of long-term immunity to B pertussis than antibody titers.

Original languageEnglish
Pages (from-to)283-289
Number of pages7
JournalArchives of Pediatrics and Adolescent Medicine
Volume151
Issue number3
Publication statusPublished - 1997

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Pertussis Vaccine
Bordetella pertussis
Cellular Immunity
Antibody Formation
Antigens
Pertussis Toxin
Vaccines
Whooping Cough
Acellular Vaccines
Vaccination
Antibodies
Hemagglutinins
Diphtheria Toxoid
Tetanus Toxoid
Diphtheria
Belgium
Tetanus
Serology
Helper-Inducer T-Lymphocytes
Italy

ASJC Scopus subject areas

  • Pediatrics, Perinatology, and Child Health

Cite this

Cossone, A., Ausiello, C. M., Urbani, F., Lande, R., Giuliano, M., Sala, A. L., ... Salmaso, S. (1997). Cell-mediated and antibody responses to bordetella pertussis antigens in children vaccinated with acellular or whole-cell pertussis vaccines. Archives of Pediatrics and Adolescent Medicine, 151(3), 283-289.

Cell-mediated and antibody responses to bordetella pertussis antigens in children vaccinated with acellular or whole-cell pertussis vaccines. / Cossone, Antonio; Ausiello, Clara M.; Urbani, Francesca; Lande, Roberta; Giuliano, Marina; Sala, Andrea La; Piscitelli, Annamaria; Salmaso, Stefania.

In: Archives of Pediatrics and Adolescent Medicine, Vol. 151, No. 3, 1997, p. 283-289.

Research output: Contribution to journalArticle

Cossone, A, Ausiello, CM, Urbani, F, Lande, R, Giuliano, M, Sala, AL, Piscitelli, A & Salmaso, S 1997, 'Cell-mediated and antibody responses to bordetella pertussis antigens in children vaccinated with acellular or whole-cell pertussis vaccines', Archives of Pediatrics and Adolescent Medicine, vol. 151, no. 3, pp. 283-289.
Cossone, Antonio ; Ausiello, Clara M. ; Urbani, Francesca ; Lande, Roberta ; Giuliano, Marina ; Sala, Andrea La ; Piscitelli, Annamaria ; Salmaso, Stefania. / Cell-mediated and antibody responses to bordetella pertussis antigens in children vaccinated with acellular or whole-cell pertussis vaccines. In: Archives of Pediatrics and Adolescent Medicine. 1997 ; Vol. 151, No. 3. pp. 283-289.
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abstract = "Objective: To examine induction and persistence of cell-mediated immunity (CMI) and antibody responses to Bordetella pertussis antigens in infants receiving antipertussis vaccines. Design and Setting: A randomized, blinded study of 142 children receiving acellular pertussis vaccines combined with diphtheria-tetanus toxoids (DTaP) (DTaP manufactured by SmithKline Beecham [DTaP-SB], Rixensart, Belgium, and DTaP manufactured by Chiron Biocin [DTaP-CB], Siena, Italy), or a whole-cell pertussis vaccine (DTwP) (Connaught Laboratories Inc, Swiftwater, Pa), or a diphtheria-tetanus (DT) (Chiron Biocine) only vaccine. Three doses of each vaccine were given at 2, 4, and 6 months of age, and CMI and antibody responses were evaluated before and at 1 and 14 months after vaccination. Methods and Main Outcome Measures: Cell- mediated immunity was assessed by proliferation of peripheral blood mononuclear cells stimulated in vitro by B pertussis antigens (pertussis toxin, filamentous hemagglutinin, and pertactin). Antibody titers against pertussis toxin, filamentous hemagglutinin, and pertactin were determined by a standardized enzyme-linked immunosorbent assay. Results: A CMI-positive response to at least 1 B pertussis antigen at 1 or both postvaccination assays was detected in 46{\%}, 55{\%}, and 83{\%} of DTwP, DTaP-SB, and DTaP-CB vaccine recipients, respectively. Frequency of CMI response to individual antigens ranged from less than 4.9{\%} against pertussis toxin in DTwP recipients to 52{\%} against pertactin in DTaP-CB recipients. The postvaccination responses measured at 14 months equalled, or had increased frequency or intensity, that of the 1-month postvaccination responses. Elevated antibody titers against the 3 antigens were present in all DTaP recipients 1 month after vaccination and were higher in CMI-positive children than in CMl-negative children. They fell, however, to low, if not negligible, levels 14 months after vaccination. Conclusions: Acellular pertussis vaccines were better inducers of CMI response than the whole-cell vaccine, particularly against pertussis toxin. Once acquired, CMI persisted, in contrast with the rapid antibody decline. Thus, CMI responses could be a useful adjunct to serology in the evaluation of pertussis vaccine immunogenicity and a better correlate of long-term immunity to B pertussis than antibody titers.",
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T1 - Cell-mediated and antibody responses to bordetella pertussis antigens in children vaccinated with acellular or whole-cell pertussis vaccines

AU - Cossone, Antonio

AU - Ausiello, Clara M.

AU - Urbani, Francesca

AU - Lande, Roberta

AU - Giuliano, Marina

AU - Sala, Andrea La

AU - Piscitelli, Annamaria

AU - Salmaso, Stefania

PY - 1997

Y1 - 1997

N2 - Objective: To examine induction and persistence of cell-mediated immunity (CMI) and antibody responses to Bordetella pertussis antigens in infants receiving antipertussis vaccines. Design and Setting: A randomized, blinded study of 142 children receiving acellular pertussis vaccines combined with diphtheria-tetanus toxoids (DTaP) (DTaP manufactured by SmithKline Beecham [DTaP-SB], Rixensart, Belgium, and DTaP manufactured by Chiron Biocin [DTaP-CB], Siena, Italy), or a whole-cell pertussis vaccine (DTwP) (Connaught Laboratories Inc, Swiftwater, Pa), or a diphtheria-tetanus (DT) (Chiron Biocine) only vaccine. Three doses of each vaccine were given at 2, 4, and 6 months of age, and CMI and antibody responses were evaluated before and at 1 and 14 months after vaccination. Methods and Main Outcome Measures: Cell- mediated immunity was assessed by proliferation of peripheral blood mononuclear cells stimulated in vitro by B pertussis antigens (pertussis toxin, filamentous hemagglutinin, and pertactin). Antibody titers against pertussis toxin, filamentous hemagglutinin, and pertactin were determined by a standardized enzyme-linked immunosorbent assay. Results: A CMI-positive response to at least 1 B pertussis antigen at 1 or both postvaccination assays was detected in 46%, 55%, and 83% of DTwP, DTaP-SB, and DTaP-CB vaccine recipients, respectively. Frequency of CMI response to individual antigens ranged from less than 4.9% against pertussis toxin in DTwP recipients to 52% against pertactin in DTaP-CB recipients. The postvaccination responses measured at 14 months equalled, or had increased frequency or intensity, that of the 1-month postvaccination responses. Elevated antibody titers against the 3 antigens were present in all DTaP recipients 1 month after vaccination and were higher in CMI-positive children than in CMl-negative children. They fell, however, to low, if not negligible, levels 14 months after vaccination. Conclusions: Acellular pertussis vaccines were better inducers of CMI response than the whole-cell vaccine, particularly against pertussis toxin. Once acquired, CMI persisted, in contrast with the rapid antibody decline. Thus, CMI responses could be a useful adjunct to serology in the evaluation of pertussis vaccine immunogenicity and a better correlate of long-term immunity to B pertussis than antibody titers.

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