Abstract
Cell-mediated immunity (CMI) to Bordetella pertussis and acellular pertussis vaccine constituents (pertussis toxin, pertactin, and filamentous hemagglutinin) were studied in peripheral blood mononuclear cells (PBMC) and T cell cultures from healthy adults with no record of vaccination against, or history of, pertussis. Similarly to stimulation with common recall antigens, PBMC proliferation was induced in 80%- 100% of the cultures, depending on the specific B. pertussis stimulant. Proliferation did not occur when antigen- presenting cells were ablated by chemical or physical methods or with naive cord blood lymphocytes. B. pertussis antigen stimulation resulted in a preferential induction of type 1 cytokine profile, as shown by interferon-γ and interleukin-2 (but no interleukin-4 or interleukin-5) gene transcripts and actual cytokine production by T cells. The data suggest that most healthy adults are repeatedly exposed to B. pertussis, with natural acquisition of antigen-specific CMI and a putatively protective type 1 cytokine pattern.
| Original language | English |
|---|---|
| Pages (from-to) | 466-470 |
| Number of pages | 5 |
| Journal | Journal of Infectious Diseases |
| Volume | 178 |
| Issue number | 2 |
| Publication status | Published - 1998 |
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ASJC Scopus subject areas
- Public Health, Environmental and Occupational Health
- Immunology
Cite this
Cell-mediated immune response of healthy adults to Bordetella pertussis vaccine antigens. / Ausiello, Clara M.; Lande, Roberto; Sala, Andrea La; Urbani, Francesca; Cassone, Antonio.
In: Journal of Infectious Diseases, Vol. 178, No. 2, 1998, p. 466-470.Research output: Contribution to journal › Article
}
TY - JOUR
T1 - Cell-mediated immune response of healthy adults to Bordetella pertussis vaccine antigens
AU - Ausiello, Clara M.
AU - Lande, Roberto
AU - Sala, Andrea La
AU - Urbani, Francesca
AU - Cassone, Antonio
PY - 1998
Y1 - 1998
N2 - Cell-mediated immunity (CMI) to Bordetella pertussis and acellular pertussis vaccine constituents (pertussis toxin, pertactin, and filamentous hemagglutinin) were studied in peripheral blood mononuclear cells (PBMC) and T cell cultures from healthy adults with no record of vaccination against, or history of, pertussis. Similarly to stimulation with common recall antigens, PBMC proliferation was induced in 80%- 100% of the cultures, depending on the specific B. pertussis stimulant. Proliferation did not occur when antigen- presenting cells were ablated by chemical or physical methods or with naive cord blood lymphocytes. B. pertussis antigen stimulation resulted in a preferential induction of type 1 cytokine profile, as shown by interferon-γ and interleukin-2 (but no interleukin-4 or interleukin-5) gene transcripts and actual cytokine production by T cells. The data suggest that most healthy adults are repeatedly exposed to B. pertussis, with natural acquisition of antigen-specific CMI and a putatively protective type 1 cytokine pattern.
AB - Cell-mediated immunity (CMI) to Bordetella pertussis and acellular pertussis vaccine constituents (pertussis toxin, pertactin, and filamentous hemagglutinin) were studied in peripheral blood mononuclear cells (PBMC) and T cell cultures from healthy adults with no record of vaccination against, or history of, pertussis. Similarly to stimulation with common recall antigens, PBMC proliferation was induced in 80%- 100% of the cultures, depending on the specific B. pertussis stimulant. Proliferation did not occur when antigen- presenting cells were ablated by chemical or physical methods or with naive cord blood lymphocytes. B. pertussis antigen stimulation resulted in a preferential induction of type 1 cytokine profile, as shown by interferon-γ and interleukin-2 (but no interleukin-4 or interleukin-5) gene transcripts and actual cytokine production by T cells. The data suggest that most healthy adults are repeatedly exposed to B. pertussis, with natural acquisition of antigen-specific CMI and a putatively protective type 1 cytokine pattern.
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UR - http://www.scopus.com/inward/citedby.url?scp=0031872473&partnerID=8YFLogxK
M3 - Article
C2 - 9697728
AN - SCOPUS:0031872473
VL - 178
SP - 466
EP - 470
JO - Journal of Infectious Diseases
JF - Journal of Infectious Diseases
SN - 0022-1899
IS - 2
ER -