Cell-mediated immune response of healthy adults to Bordetella pertussis vaccine antigens

Clara M. Ausiello, Roberto Lande, Andrea La Sala, Francesca Urbani, Antonio Cassone

Research output: Contribution to journalArticle

30 Citations (Scopus)

Abstract

Cell-mediated immunity (CMI) to Bordetella pertussis and acellular pertussis vaccine constituents (pertussis toxin, pertactin, and filamentous hemagglutinin) were studied in peripheral blood mononuclear cells (PBMC) and T cell cultures from healthy adults with no record of vaccination against, or history of, pertussis. Similarly to stimulation with common recall antigens, PBMC proliferation was induced in 80%- 100% of the cultures, depending on the specific B. pertussis stimulant. Proliferation did not occur when antigen- presenting cells were ablated by chemical or physical methods or with naive cord blood lymphocytes. B. pertussis antigen stimulation resulted in a preferential induction of type 1 cytokine profile, as shown by interferon-γ and interleukin-2 (but no interleukin-4 or interleukin-5) gene transcripts and actual cytokine production by T cells. The data suggest that most healthy adults are repeatedly exposed to B. pertussis, with natural acquisition of antigen-specific CMI and a putatively protective type 1 cytokine pattern.

Original languageEnglish
Pages (from-to)466-470
Number of pages5
JournalJournal of Infectious Diseases
Volume178
Issue number2
Publication statusPublished - 1998

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Pertussis Vaccine
Bordetella pertussis
Antigens
Cytokines
Cellular Immunity
Blood Cells
Acellular Vaccines
T-Lymphocytes
Whooping Cough
Interleukin-5
Pertussis Toxin
Hemagglutinins
Antigen-Presenting Cells
Fetal Blood
Interleukin-4
Interferons
Interleukin-2
Vaccination
Cell Culture Techniques
Cell Proliferation

ASJC Scopus subject areas

  • Public Health, Environmental and Occupational Health
  • Immunology

Cite this

Ausiello, C. M., Lande, R., Sala, A. L., Urbani, F., & Cassone, A. (1998). Cell-mediated immune response of healthy adults to Bordetella pertussis vaccine antigens. Journal of Infectious Diseases, 178(2), 466-470.

Cell-mediated immune response of healthy adults to Bordetella pertussis vaccine antigens. / Ausiello, Clara M.; Lande, Roberto; Sala, Andrea La; Urbani, Francesca; Cassone, Antonio.

In: Journal of Infectious Diseases, Vol. 178, No. 2, 1998, p. 466-470.

Research output: Contribution to journalArticle

Ausiello, CM, Lande, R, Sala, AL, Urbani, F & Cassone, A 1998, 'Cell-mediated immune response of healthy adults to Bordetella pertussis vaccine antigens', Journal of Infectious Diseases, vol. 178, no. 2, pp. 466-470.
Ausiello, Clara M. ; Lande, Roberto ; Sala, Andrea La ; Urbani, Francesca ; Cassone, Antonio. / Cell-mediated immune response of healthy adults to Bordetella pertussis vaccine antigens. In: Journal of Infectious Diseases. 1998 ; Vol. 178, No. 2. pp. 466-470.
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