Cell membrane expression and functional role of the p75 subunit of interleukin-2 receptor in lymphoproliferative disease of granular lymphocytes

Renato Zambello, Livio Trentin, Giovanni Pizzolo, Pietro Bulian, Maria Masciarelli, Cristina Feruglio, Carlo Agostini, Roberto Raimondi, Teodoro Chisesi, Gianpietro Semenzato

Research output: Contribution to journalArticle

Abstract

The cell membrane expression and functional role of the interleukin-2 receptor (IL-2R) was analyzed in nine patients with lymphoproliferative disease of granular lymphocytes (LDGL) using monoclonal antibodies (MoAbs) specific for the p75 (TU27) and the p55 (anti-Tac) subunits of IL-2R. Four patients were characterized by the proliferation of CD3+CD8+ granular lymphocytes (GL) expressing the α/β T-cell receptor (Tα/β) and one case by the proliferation of CD3+CD4-CD8- GL expressing the γ/δ T-cell receptor (Tγδ); in four additional cases proliferating cells were CD3 negative GL. Consistent with data observed on normal GL, phenotypic analysis demonstrated that patients' GL lack the expression of the p55 IL-2R, whereas the p75 subunit is constitutionally expressed by expanding GL of both T-cell (either Tαβ and Tγδ) and natural killer (NK) origin in variable proportions (11% to 94% of cells). The analysis of the cytotoxic and proliferative activity demonstrated that the anti-p55 MoAb failed to inhibit IL-2-mediated activation, whereas a marked inhibition of both cytotoxicity and proliferation were obtained using the anti-p75 chain specific MoAb. These data indicate that the p75 chain of IL-2R is responsible for IL-2 signal transduction in both CDS+ and CD3- LDGL patients' GL.

Original languageEnglish
Pages (from-to)2080-2085
Number of pages6
JournalBlood
Volume76
Issue number10
Publication statusPublished - Nov 15 1990

ASJC Scopus subject areas

  • Hematology

Fingerprint Dive into the research topics of 'Cell membrane expression and functional role of the p75 subunit of interleukin-2 receptor in lymphoproliferative disease of granular lymphocytes'. Together they form a unique fingerprint.

  • Cite this

    Zambello, R., Trentin, L., Pizzolo, G., Bulian, P., Masciarelli, M., Feruglio, C., Agostini, C., Raimondi, R., Chisesi, T., & Semenzato, G. (1990). Cell membrane expression and functional role of the p75 subunit of interleukin-2 receptor in lymphoproliferative disease of granular lymphocytes. Blood, 76(10), 2080-2085.