Abstract
The cell membrane expression and functional role of the interleukin-2 receptor (IL-2R) was analyzed in nine patients with lymphoproliferative disease of granular lymphocytes (LDGL) using monoclonal antibodies (MoAbs) specific for the p75 (TU27) and the p55 (anti-Tac) subunits of IL-2R. Four patients were characterized by the proliferation of CD3+CD8+ granular lymphocytes (GL) expressing the α/β T-cell receptor (Tα/β) and one case by the proliferation of CD3+CD4-CD8- GL expressing the γ/δ T-cell receptor (Tγδ); in four additional cases proliferating cells were CD3 negative GL. Consistent with data observed on normal GL, phenotypic analysis demonstrated that patients' GL lack the expression of the p55 IL-2R, whereas the p75 subunit is constitutionally expressed by expanding GL of both T-cell (either Tαβ and Tγδ) and natural killer (NK) origin in variable proportions (11% to 94% of cells). The analysis of the cytotoxic and proliferative activity demonstrated that the anti-p55 MoAb failed to inhibit IL-2-mediated activation, whereas a marked inhibition of both cytotoxicity and proliferation were obtained using the anti-p75 chain specific MoAb. These data indicate that the p75 chain of IL-2R is responsible for IL-2 signal transduction in both CDS+ and CD3- LDGL patients' GL.
| Original language | English |
|---|---|
| Pages (from-to) | 2080-2085 |
| Number of pages | 6 |
| Journal | Blood |
| Volume | 76 |
| Issue number | 10 |
| Publication status | Published - Nov 15 1990 |
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ASJC Scopus subject areas
- Hematology
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Cell membrane expression and functional role of the p75 subunit of interleukin-2 receptor in lymphoproliferative disease of granular lymphocytes. / Zambello, Renato; Trentin, Livio; Pizzolo, Giovanni; Bulian, Pietro; Masciarelli, Maria; Feruglio, Cristina; Agostini, Carlo; Raimondi, Roberto; Chisesi, Teodoro; Semenzato, Gianpietro.
In: Blood, Vol. 76, No. 10, 15.11.1990, p. 2080-2085.Research output: Contribution to journal › Article
}
TY - JOUR
T1 - Cell membrane expression and functional role of the p75 subunit of interleukin-2 receptor in lymphoproliferative disease of granular lymphocytes
AU - Zambello, Renato
AU - Trentin, Livio
AU - Pizzolo, Giovanni
AU - Bulian, Pietro
AU - Masciarelli, Maria
AU - Feruglio, Cristina
AU - Agostini, Carlo
AU - Raimondi, Roberto
AU - Chisesi, Teodoro
AU - Semenzato, Gianpietro
PY - 1990/11/15
Y1 - 1990/11/15
N2 - The cell membrane expression and functional role of the interleukin-2 receptor (IL-2R) was analyzed in nine patients with lymphoproliferative disease of granular lymphocytes (LDGL) using monoclonal antibodies (MoAbs) specific for the p75 (TU27) and the p55 (anti-Tac) subunits of IL-2R. Four patients were characterized by the proliferation of CD3+CD8+ granular lymphocytes (GL) expressing the α/β T-cell receptor (Tα/β) and one case by the proliferation of CD3+CD4-CD8- GL expressing the γ/δ T-cell receptor (Tγδ); in four additional cases proliferating cells were CD3 negative GL. Consistent with data observed on normal GL, phenotypic analysis demonstrated that patients' GL lack the expression of the p55 IL-2R, whereas the p75 subunit is constitutionally expressed by expanding GL of both T-cell (either Tαβ and Tγδ) and natural killer (NK) origin in variable proportions (11% to 94% of cells). The analysis of the cytotoxic and proliferative activity demonstrated that the anti-p55 MoAb failed to inhibit IL-2-mediated activation, whereas a marked inhibition of both cytotoxicity and proliferation were obtained using the anti-p75 chain specific MoAb. These data indicate that the p75 chain of IL-2R is responsible for IL-2 signal transduction in both CDS+ and CD3- LDGL patients' GL.
AB - The cell membrane expression and functional role of the interleukin-2 receptor (IL-2R) was analyzed in nine patients with lymphoproliferative disease of granular lymphocytes (LDGL) using monoclonal antibodies (MoAbs) specific for the p75 (TU27) and the p55 (anti-Tac) subunits of IL-2R. Four patients were characterized by the proliferation of CD3+CD8+ granular lymphocytes (GL) expressing the α/β T-cell receptor (Tα/β) and one case by the proliferation of CD3+CD4-CD8- GL expressing the γ/δ T-cell receptor (Tγδ); in four additional cases proliferating cells were CD3 negative GL. Consistent with data observed on normal GL, phenotypic analysis demonstrated that patients' GL lack the expression of the p55 IL-2R, whereas the p75 subunit is constitutionally expressed by expanding GL of both T-cell (either Tαβ and Tγδ) and natural killer (NK) origin in variable proportions (11% to 94% of cells). The analysis of the cytotoxic and proliferative activity demonstrated that the anti-p55 MoAb failed to inhibit IL-2-mediated activation, whereas a marked inhibition of both cytotoxicity and proliferation were obtained using the anti-p75 chain specific MoAb. These data indicate that the p75 chain of IL-2R is responsible for IL-2 signal transduction in both CDS+ and CD3- LDGL patients' GL.
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M3 - Article
C2 - 2146981
AN - SCOPUS:0025202073
VL - 76
SP - 2080
EP - 2085
JO - Blood
JF - Blood
SN - 0006-4971
IS - 10
ER -