Cell membrane expression and functional role of the p75 subunit of interleukin-2 receptor in lymphoproliferative disease of granular lymphocytes

Renato Zambello, Livio Trentin, Giovanni Pizzolo, Pietro Bulian, Maria Masciarelli, Cristina Feruglio, Carlo Agostini, Roberto Raimondi, Teodoro Chisesi, Gianpietro Semenzato

Research output: Contribution to journalArticle

Abstract

The cell membrane expression and functional role of the interleukin-2 receptor (IL-2R) was analyzed in nine patients with lymphoproliferative disease of granular lymphocytes (LDGL) using monoclonal antibodies (MoAbs) specific for the p75 (TU27) and the p55 (anti-Tac) subunits of IL-2R. Four patients were characterized by the proliferation of CD3+CD8+ granular lymphocytes (GL) expressing the α/β T-cell receptor (Tα/β) and one case by the proliferation of CD3+CD4-CD8- GL expressing the γ/δ T-cell receptor (Tγδ); in four additional cases proliferating cells were CD3 negative GL. Consistent with data observed on normal GL, phenotypic analysis demonstrated that patients' GL lack the expression of the p55 IL-2R, whereas the p75 subunit is constitutionally expressed by expanding GL of both T-cell (either Tαβ and Tγδ) and natural killer (NK) origin in variable proportions (11% to 94% of cells). The analysis of the cytotoxic and proliferative activity demonstrated that the anti-p55 MoAb failed to inhibit IL-2-mediated activation, whereas a marked inhibition of both cytotoxicity and proliferation were obtained using the anti-p75 chain specific MoAb. These data indicate that the p75 chain of IL-2R is responsible for IL-2 signal transduction in both CDS+ and CD3- LDGL patients' GL.

Original languageEnglish
Pages (from-to)2080-2085
Number of pages6
JournalBlood
Volume76
Issue number10
Publication statusPublished - Nov 15 1990

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Large Granular Lymphocytic Leukemia
Lymphocytes
Interleukin-2 Receptors
Cell membranes
Cell Membrane
T-Cell Antigen Receptor
Interleukin-2
Signal transduction
T-cells
Signal Transduction
Cytotoxicity
Monoclonal Antibodies
T-Lymphocytes
Chemical activation

ASJC Scopus subject areas

  • Hematology

Cite this

Zambello, R., Trentin, L., Pizzolo, G., Bulian, P., Masciarelli, M., Feruglio, C., ... Semenzato, G. (1990). Cell membrane expression and functional role of the p75 subunit of interleukin-2 receptor in lymphoproliferative disease of granular lymphocytes. Blood, 76(10), 2080-2085.

Cell membrane expression and functional role of the p75 subunit of interleukin-2 receptor in lymphoproliferative disease of granular lymphocytes. / Zambello, Renato; Trentin, Livio; Pizzolo, Giovanni; Bulian, Pietro; Masciarelli, Maria; Feruglio, Cristina; Agostini, Carlo; Raimondi, Roberto; Chisesi, Teodoro; Semenzato, Gianpietro.

In: Blood, Vol. 76, No. 10, 15.11.1990, p. 2080-2085.

Research output: Contribution to journalArticle

Zambello, R, Trentin, L, Pizzolo, G, Bulian, P, Masciarelli, M, Feruglio, C, Agostini, C, Raimondi, R, Chisesi, T & Semenzato, G 1990, 'Cell membrane expression and functional role of the p75 subunit of interleukin-2 receptor in lymphoproliferative disease of granular lymphocytes', Blood, vol. 76, no. 10, pp. 2080-2085.
Zambello, Renato ; Trentin, Livio ; Pizzolo, Giovanni ; Bulian, Pietro ; Masciarelli, Maria ; Feruglio, Cristina ; Agostini, Carlo ; Raimondi, Roberto ; Chisesi, Teodoro ; Semenzato, Gianpietro. / Cell membrane expression and functional role of the p75 subunit of interleukin-2 receptor in lymphoproliferative disease of granular lymphocytes. In: Blood. 1990 ; Vol. 76, No. 10. pp. 2080-2085.
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AU - Feruglio, Cristina

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