Cell motility is controlled by SF2/ASF through alternative splicing of the Ron protooncogene

Claudia Ghigna, Silvia Giordano, Haihong Shen, Federica Benvenuto, Fabio Castiglioni, Paolo Maria Comoglio, Michael R. Green, Silvano Riva, Giuseppe Biamonti

Research output: Contribution to journalArticlepeer-review


Ron, the tyrosine kinase receptor for the Macrophage-stimulating protein, is involved in cell dissociation, motility, and matrix invasion. ΔRon, a constitutively active isoform that confers increased motility to expressing cells, is generated through the skipping of exon 11. We show that abnormal accumulation of ΔRon mRNA occurs in breast and colon tumors. Skipping of exon 11 is controlled by a silencer and an enhancer of splicing located in the constitutive exon 12. The strength of the enhancer parallels the relative abundance of ΔRon mRNA and depends on a sequence directly bound by splicing factor SF2/ASF. Overexpression and RNAi experiments demonstrate that SF2/ASF, by controlling the production of ΔRon, activates epithelial to mesenchymal transition leading to cell locomotion. The effect of SF2/ASF overexpression is reverted by specific knockdown of ΔRon mRNA. This demonstrates a direct link between SF2/ASF-regulated splicing and cell motility, an activity important for embryogenesis, tissue formation, and tumor metastasis.

Original languageEnglish
Pages (from-to)881-890
Number of pages10
JournalMolecular Cell
Issue number6
Publication statusPublished - Dec 22 2005

ASJC Scopus subject areas

  • Molecular Biology


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