Cell proliferation curves of different human tumor lines after in vitro treatment with zebrafish embryonic extracts

P. M. Biava, D. Bonsignorio, M. Hoxha

Research output: Contribution to journalArticle


Five tumor cell lines of different origin (glioblastoma, melanoma, kidney adenocarcinorna, breast carcinoma and lymphoblastic leukemia) were treated in vitro with the extracts from zebrafish embryos collected at four different developmental stages. All cell lines responded with a significant slowing of the proliferation when treated with the extracts taken during the stages of cell differentiation, while no slowing effect was observed when they were treated with the extract taken from a merely multiplicative stage. These results suggest that a complex network of molecular factors during embryo differentiation may help abnormally proliferating cells to normalize their cycle, and that the administration of embryonic cell differentiation factors may be a useful tool in cancer therapy. On the other hand, it is known that the stem cells can be differentiated into different types of cells in relationship to different kinds of embryonic microenvironment. Since this network of cell differentiation factors may normalize the altered expression of genes, we suggest it as a sort of physiological gene therapy.

Original languageEnglish
Pages (from-to)195-201
Number of pages7
JournalJournal of Tumor Marker Oncology
Issue number3
Publication statusPublished - 2001


ASJC Scopus subject areas

  • Cancer Research

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