TY - JOUR
T1 - Cell proliferation in developing human dental pulp
T2 - A combined flow cytometric and immunohistochemical study
AU - Casasco, Andrea
AU - Casasco, Marco
AU - Calligaro, Alberto
AU - Ferrieri, Giovanni
AU - Brambilla, Eugenio
AU - Strohmenger, Laura
AU - Alberici, Roberta
AU - Mazzini, Giuliano
PY - 1997/12
Y1 - 1997/12
N2 - Information concerning cell proliferation and differentiation in dental pulp may be important to understand tooth response to exogenous stimuli. Since few data concerning human dental pulp are available, we have investigated the growth fraction and the localization of proliferating cells in pulp tissue of third molars of young adult human males and females, using flow cytometry and immunohistochemistry. Flow cytometric analysis demonstrates a low proliferative activity of pulp tissue that appears to be confined to radicular pulp, as revealed by immunohistochemical detection of proliferating cells. No polyploid or aneuploid cell populations could be identified, and G2-blocked cells, if any, represented a negligible cell population. Odontoblasts, cells of the sub-odontoblastic layer, and cells of coronal pulp were found to be not proliferating under normal conditions. These data provide the basis for future investigations on proliferative activity and regenerative potentiality of human pulp cells in experimental and clinical situations.
AB - Information concerning cell proliferation and differentiation in dental pulp may be important to understand tooth response to exogenous stimuli. Since few data concerning human dental pulp are available, we have investigated the growth fraction and the localization of proliferating cells in pulp tissue of third molars of young adult human males and females, using flow cytometry and immunohistochemistry. Flow cytometric analysis demonstrates a low proliferative activity of pulp tissue that appears to be confined to radicular pulp, as revealed by immunohistochemical detection of proliferating cells. No polyploid or aneuploid cell populations could be identified, and G2-blocked cells, if any, represented a negligible cell population. Odontoblasts, cells of the sub-odontoblastic layer, and cells of coronal pulp were found to be not proliferating under normal conditions. These data provide the basis for future investigations on proliferative activity and regenerative potentiality of human pulp cells in experimental and clinical situations.
KW - Dental pulp
KW - Flow cytometry
KW - Human
KW - Immunohistochemistry
KW - Proliferation
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M3 - Article
C2 - 9469613
AN - SCOPUS:0031299391
VL - 105
SP - 609
EP - 613
JO - European Journal of Oral Sciences
JF - European Journal of Oral Sciences
SN - 0909-8836
IS - 6
ER -