Cell proliferation in developing human dental pulp: A combined flow cytometric and immunohistochemical study

Andrea Casasco, Marco Casasco, Alberto Calligaro, Giovanni Ferrieri, Eugenio Brambilla, Laura Strohmenger, Roberta Alberici, Giuliano Mazzini

Research output: Contribution to journalArticlepeer-review

Abstract

Information concerning cell proliferation and differentiation in dental pulp may be important to understand tooth response to exogenous stimuli. Since few data concerning human dental pulp are available, we have investigated the growth fraction and the localization of proliferating cells in pulp tissue of third molars of young adult human males and females, using flow cytometry and immunohistochemistry. Flow cytometric analysis demonstrates a low proliferative activity of pulp tissue that appears to be confined to radicular pulp, as revealed by immunohistochemical detection of proliferating cells. No polyploid or aneuploid cell populations could be identified, and G2-blocked cells, if any, represented a negligible cell population. Odontoblasts, cells of the sub-odontoblastic layer, and cells of coronal pulp were found to be not proliferating under normal conditions. These data provide the basis for future investigations on proliferative activity and regenerative potentiality of human pulp cells in experimental and clinical situations.

Original languageEnglish
Pages (from-to)609-613
Number of pages5
JournalEuropean Journal of Oral Sciences
Volume105
Issue number6
Publication statusPublished - Dec 1997

Keywords

  • Dental pulp
  • Flow cytometry
  • Human
  • Immunohistochemistry
  • Proliferation

ASJC Scopus subject areas

  • Dentistry(all)

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