TY - JOUR
T1 - Cell proliferation of squamous epithelium in gastro-oesophageal reflux disease
T2 - Correlations with clinical, endoscopic and morphological data
AU - Mastracci, L.
AU - Grillo, F.
AU - Zentilin, P.
AU - Spaggiari, P.
AU - Dulbecco, P.
AU - Pigozzi, S.
AU - Savarino, V.
AU - Fiocca, R.
PY - 2007/3
Y1 - 2007/3
N2 - Background: The microscopic assessment of squamous epithelium lesions in gastro-oesophageal reflux disease (GERD) is subjective. The Ki67 nuclear antigen expressed by proliferating cells provides an objective measure of regeneration in the squamous epithelium. Aim: To evaluate Ki67 expression in GERD patients and controls, in comparison with histological lesions, pH-metry and endoscopic data. Methods: Eighty-seven patients with GERD symptoms and 20 symptom-free controls underwent endoscopy and 24-h pH monitoring. Oesophageal biopsies (4 cm, 2 cm and Z-line) were stained with Ki67/MIB-1 antibodies; the Ki67-positive nuclear area was assessed with an image analysis system and expressed as percentage of the whole epithelial area (Ki67-%). Results: Ki67-% was significantly higher in 32 patients with erosive oesophagitis, 44 endoscopy-negative GERD and 11 patients with functional heartburn than in controls (P = 0.0001). Both controls and patients showed a progressive increase in Ki67-% from 4 cm to the Z-line (P <0.0001). Ki67-% showed a significant correlation with other conventional histological lesions (P ranged between 0.0151 and
AB - Background: The microscopic assessment of squamous epithelium lesions in gastro-oesophageal reflux disease (GERD) is subjective. The Ki67 nuclear antigen expressed by proliferating cells provides an objective measure of regeneration in the squamous epithelium. Aim: To evaluate Ki67 expression in GERD patients and controls, in comparison with histological lesions, pH-metry and endoscopic data. Methods: Eighty-seven patients with GERD symptoms and 20 symptom-free controls underwent endoscopy and 24-h pH monitoring. Oesophageal biopsies (4 cm, 2 cm and Z-line) were stained with Ki67/MIB-1 antibodies; the Ki67-positive nuclear area was assessed with an image analysis system and expressed as percentage of the whole epithelial area (Ki67-%). Results: Ki67-% was significantly higher in 32 patients with erosive oesophagitis, 44 endoscopy-negative GERD and 11 patients with functional heartburn than in controls (P = 0.0001). Both controls and patients showed a progressive increase in Ki67-% from 4 cm to the Z-line (P <0.0001). Ki67-% showed a significant correlation with other conventional histological lesions (P ranged between 0.0151 and
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U2 - 10.1111/j.1365-2036.2006.03243.x
DO - 10.1111/j.1365-2036.2006.03243.x
M3 - Article
C2 - 17305765
AN - SCOPUS:33846969845
VL - 25
SP - 637
EP - 645
JO - Alimentary Pharmacology and Therapeutics
JF - Alimentary Pharmacology and Therapeutics
SN - 0269-2813
IS - 5
ER -