Cell surface antigens of chemically induced fibrosarcomas: Detection by a monoclonal antibody of a tumor-restricted M(r) 12,000 protein gag antigen encoded by a dual-tropic murine leukemia virus

G. Carbone, M. A. Pierotti, M. Boiocchi, A. B. De Leo, D. Ballinari, P. Radice, M. G. Borrello, A. Meseguer, A. Cernuschi, G. Parmiani

Research output: Contribution to journalArticle

Abstract

Fusion products of spleen cells of W/FuDp rats immunized with a methylcholanthrene-induced BALB/c sarcoma, CA-2, and mouse myeloma cells were screened in an attempt to identify a monoclonal antibody defining the individually distinct tumor-specific transplantation antigen of CA-2. A hybridoma, MP/69/04, was isolated which produces an IgG(2a) monoclonal antibody that recognized a tumor-restricted antigen of CA-2. In direct binding assay, MP/69/04 reacted only with 2 of 15 methylcholanthrene induced BALB/c sarcomas tested. Thymus, spleen, lymph nodes, bone marrow, brain, adult lung fibroblasts, newborn muscle fibroblasts and 3T3 cells were negative. Absorption tests revealed, however, expression of the MP/69/04 determinant on 8 of the 12 murine leukemia virus (MuLV) producer BALB/c sarcoma tested. The antigen was not detected on any of the three non-producer sarcomas tested nor on a wide range of normal tissues and cell lines. An N-dualtropic MuLV was isolated from CA-2, and cell lines susceptible to infection by this virus were shown to express the MP/69/04 epitope. By Western blotting, the MP/69/04 epitope was identified as being expressed on the MuLV structural protein with a molecular weight of 12,000, present in CA-2 cells and in the purified CA-2 MuLV. These results indicate the MP/69/04 antigen is not a unique tumor-specific transplantation antigen but is a gag product of a recombinant retrovirus which is expressed on the cell surface of many MuLV + methylcholanthrene-induced BALB/c fibrosarcomas.

Original languageEnglish
Pages (from-to)4980-4985
Number of pages6
JournalCancer Research
Volume45
Issue number10
Publication statusPublished - 1985

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gag Gene Products
Murine Leukemia Viruses
Fibrosarcoma
Surface Antigens
Sarcoma
Monoclonal Antibodies
Methylcholanthrene
Neoplasms
Proteins
Epitopes
Spleen
Fibroblasts
Viral Structural Proteins
Antigens
Cell Line
3T3 Cells
Hybridomas
Neoplasm Antigens
Virus Diseases
Retroviridae

ASJC Scopus subject areas

  • Cancer Research
  • Oncology

Cite this

Cell surface antigens of chemically induced fibrosarcomas : Detection by a monoclonal antibody of a tumor-restricted M(r) 12,000 protein gag antigen encoded by a dual-tropic murine leukemia virus. / Carbone, G.; Pierotti, M. A.; Boiocchi, M.; De Leo, A. B.; Ballinari, D.; Radice, P.; Borrello, M. G.; Meseguer, A.; Cernuschi, A.; Parmiani, G.

In: Cancer Research, Vol. 45, No. 10, 1985, p. 4980-4985.

Research output: Contribution to journalArticle

Carbone, G. ; Pierotti, M. A. ; Boiocchi, M. ; De Leo, A. B. ; Ballinari, D. ; Radice, P. ; Borrello, M. G. ; Meseguer, A. ; Cernuschi, A. ; Parmiani, G. / Cell surface antigens of chemically induced fibrosarcomas : Detection by a monoclonal antibody of a tumor-restricted M(r) 12,000 protein gag antigen encoded by a dual-tropic murine leukemia virus. In: Cancer Research. 1985 ; Vol. 45, No. 10. pp. 4980-4985.
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abstract = "Fusion products of spleen cells of W/FuDp rats immunized with a methylcholanthrene-induced BALB/c sarcoma, CA-2, and mouse myeloma cells were screened in an attempt to identify a monoclonal antibody defining the individually distinct tumor-specific transplantation antigen of CA-2. A hybridoma, MP/69/04, was isolated which produces an IgG(2a) monoclonal antibody that recognized a tumor-restricted antigen of CA-2. In direct binding assay, MP/69/04 reacted only with 2 of 15 methylcholanthrene induced BALB/c sarcomas tested. Thymus, spleen, lymph nodes, bone marrow, brain, adult lung fibroblasts, newborn muscle fibroblasts and 3T3 cells were negative. Absorption tests revealed, however, expression of the MP/69/04 determinant on 8 of the 12 murine leukemia virus (MuLV) producer BALB/c sarcoma tested. The antigen was not detected on any of the three non-producer sarcomas tested nor on a wide range of normal tissues and cell lines. An N-dualtropic MuLV was isolated from CA-2, and cell lines susceptible to infection by this virus were shown to express the MP/69/04 epitope. By Western blotting, the MP/69/04 epitope was identified as being expressed on the MuLV structural protein with a molecular weight of 12,000, present in CA-2 cells and in the purified CA-2 MuLV. These results indicate the MP/69/04 antigen is not a unique tumor-specific transplantation antigen but is a gag product of a recombinant retrovirus which is expressed on the cell surface of many MuLV + methylcholanthrene-induced BALB/c fibrosarcomas.",
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T2 - Detection by a monoclonal antibody of a tumor-restricted M(r) 12,000 protein gag antigen encoded by a dual-tropic murine leukemia virus

AU - Carbone, G.

AU - Pierotti, M. A.

AU - Boiocchi, M.

AU - De Leo, A. B.

AU - Ballinari, D.

AU - Radice, P.

AU - Borrello, M. G.

AU - Meseguer, A.

AU - Cernuschi, A.

AU - Parmiani, G.

PY - 1985

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