Cellular and molecular analyses of Interferon β cytopathic effect on HPV-16 in vitro transformed human keratinocytes (HPK-IA)

F. De Marco, M. L. Marcante

Research output: Contribution to journalArticle

3 Citations (Scopus)

Abstract

Recently, Interferon (IFN) β has been reported to induce on HPK-IA cell line, an unusually marked cytopathic effect. Surprisingly this effect is not shared with IFN a. The morphological aspect of this cytopathic effect recals the one induced by highly cytopathic viruses. It has an induction time of less then four hours, may be inhibited by protein synthesis inhibitors and has a characteristic protein SDS-PAGE pattern. RT-PCR analysis has shown that IFNβ, but not α or γ, reduces the transcription level of E6/E7 viral mRNA. Taken together these data suggest that cells may finely discriminate between IFNα and β. Regarding HPK-IA cells this discrimination allows the activation of a specific transduction pathway ultimately leading to cell death. Cell death may be due to the concomitant severe reduction of E6/E7 viral transcription observed.

Original languageEnglish
Pages (from-to)24-30
Number of pages7
JournalJournal of Biological Regulators and Homeostatic Agents
Volume9
Issue number1
Publication statusPublished - 1995

Fingerprint

Human papillomavirus 16
cytopathogenicity
keratinocytes
interferons
Keratinocytes
Interferons
cell death
Cell Death
transcription (genetics)
protein synthesis inhibitors
Protein Synthesis Inhibitors
Polyacrylamide Gel Electrophoresis
reverse transcriptase polymerase chain reaction
cell lines
cells
Viruses
Cell Line
Polymerase Chain Reaction
viruses
Messenger RNA

Keywords

  • Cytopathic effect
  • E6
  • E7
  • Human papillomavirus type 16
  • Interferon β

ASJC Scopus subject areas

  • Agricultural and Biological Sciences(all)
  • Endocrinology
  • Physiology
  • Immunology
  • Medicine (miscellaneous)
  • Endocrinology, Diabetes and Metabolism
  • Physiology (medical)

Cite this

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AB - Recently, Interferon (IFN) β has been reported to induce on HPK-IA cell line, an unusually marked cytopathic effect. Surprisingly this effect is not shared with IFN a. The morphological aspect of this cytopathic effect recals the one induced by highly cytopathic viruses. It has an induction time of less then four hours, may be inhibited by protein synthesis inhibitors and has a characteristic protein SDS-PAGE pattern. RT-PCR analysis has shown that IFNβ, but not α or γ, reduces the transcription level of E6/E7 viral mRNA. Taken together these data suggest that cells may finely discriminate between IFNα and β. Regarding HPK-IA cells this discrimination allows the activation of a specific transduction pathway ultimately leading to cell death. Cell death may be due to the concomitant severe reduction of E6/E7 viral transcription observed.

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