TY - JOUR
T1 - Cellular and subcellular localization of uncoupling protein 2 in the human kidney
AU - Nigro, Michelangelo
AU - De Sanctis, Claudia
AU - Formisano, Pietro
AU - Stanzione, Rosita
AU - Forte, Maurizio
AU - Capasso, Giovambattista
AU - Gigliotti, Giuseppe
AU - Rubattu, Speranza
AU - Viggiano, Davide
PY - 2018/8/1
Y1 - 2018/8/1
N2 - The uncoupling protein-2 (UCP2) is an anion transporter that plays a key role in the control of intracellular oxidative stress. In animal models UCP2 downregulation has several pathological sequelae, particularly affecting the vasculature and the kidney. Specifically, in these models kidney damage is highly favored in the absence of UCP2 in the context of experimental hypertension. Confirmations of these data in humans awaits further information, as no data are yet available concerning the cell-type and subcellular expression in the human kidney. In the present study, we aimed to characterize the UCP2 protein distribution in human kidney biopsies. In humans UCP2 is mainly localized in proximal convoluted tubule cells, with an intracytoplasmic punctate staining. UCP2 positive puncta are often localized at the interface between the endoplasmic reticulum and the mitochondria. Glomerular structures do not express UCP2 at detectable levels. The expression of UCP2 in proximal tubular cells may explain their relative propensity to damage in pathological conditions including the hypertensive disease.
AB - The uncoupling protein-2 (UCP2) is an anion transporter that plays a key role in the control of intracellular oxidative stress. In animal models UCP2 downregulation has several pathological sequelae, particularly affecting the vasculature and the kidney. Specifically, in these models kidney damage is highly favored in the absence of UCP2 in the context of experimental hypertension. Confirmations of these data in humans awaits further information, as no data are yet available concerning the cell-type and subcellular expression in the human kidney. In the present study, we aimed to characterize the UCP2 protein distribution in human kidney biopsies. In humans UCP2 is mainly localized in proximal convoluted tubule cells, with an intracytoplasmic punctate staining. UCP2 positive puncta are often localized at the interface between the endoplasmic reticulum and the mitochondria. Glomerular structures do not express UCP2 at detectable levels. The expression of UCP2 in proximal tubular cells may explain their relative propensity to damage in pathological conditions including the hypertensive disease.
KW - Human kidney
KW - Hypertension
KW - Mitochondria
KW - Renal proximal tubular epithelial cells
KW - Uncoupling proteins
UR - http://www.scopus.com/inward/record.url?scp=85048967487&partnerID=8YFLogxK
UR - http://www.scopus.com/inward/citedby.url?scp=85048967487&partnerID=8YFLogxK
U2 - 10.1007/s10735-018-9782-3
DO - 10.1007/s10735-018-9782-3
M3 - Article
AN - SCOPUS:85048967487
VL - 49
SP - 437
EP - 445
JO - Journal of Molecular Histology
JF - Journal of Molecular Histology
SN - 1567-2379
IS - 4
ER -