Cellular distribution and degradation of cobalt ferrite nanoparticles in Balb/3T3 mouse fibroblasts

Patrick Marmorato; Giacomo Ceccone; Alessandra Gianoncelli; Lorella Pascolo; Jessica Ponti; François Rossi; Murielle Salomé; Burkhard Kaulich; Maya Kiskinova

doi:10.1016/j.toxlet.2011.08.026

Cellular distribution and degradation of cobalt ferrite nanoparticles in Balb/3T3 mouse fibroblasts

Patrick Marmorato, Giacomo Ceccone, Alessandra Gianoncelli, Lorella Pascolo, Jessica Ponti, François Rossi, Murielle Salomé, Burkhard Kaulich, Maya Kiskinova

IRCCS pediatrico Burlo Garofolo

Research output: Contribution to journal › Article › peer-review

Abstract

The effect of the concentration of cobalt ferrite (CoFe ₂O ₄) nanoparticles (NPs) on their intracellular location and distribution has been explored by synchrotron radiation X-ray and fluorescence microscopy (SR-XRF) monitoring the evolution of NPs elemental composition as well. In cells exposed to low concentrations of CoFe ₂O ₄ NPs, the NPs preferentially segregate in the perinuclear region preserving their initial chemical content. At concentrations exceeding 500μM the XRF spectra indicate the presence of Co and Fe also in the nuclear region, accompanied by sensible changes in the cellular morphology. The increase of the Co/Fe ratio measured in the nuclear compartment indicates that above certain concentrations the CoFe ₂O ₄ NPs intracellular distribution could be accompanied by biodegradation resulting in Co accumulation in the nucleus.

Original language	English
Pages (from-to)	128-136
Number of pages	9
Journal	Toxicology Letters
Volume	207
Issue number	2
DOIs	https://doi.org/10.1016/j.toxlet.2011.08.026
Publication status	Published - Nov 30 2011

Keywords

Fibroblasts
Magnetic nanoparticles
X-ray fluorescence
X-ray microscopy

ASJC Scopus subject areas

Toxicology

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10.1016/j.toxlet.2011.08.026

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Cellular distribution and degradation of cobalt ferrite nanoparticles in Balb/3T3 mouse fibroblasts. / Marmorato, Patrick; Ceccone, Giacomo; Gianoncelli, Alessandra; Pascolo, Lorella; Ponti, Jessica; Rossi, François; Salomé, Murielle; Kaulich, Burkhard; Kiskinova, Maya.

In: Toxicology Letters, Vol. 207, No. 2, 30.11.2011, p. 128-136.

Research output: Contribution to journal › Article › peer-review

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title = "Cellular distribution and degradation of cobalt ferrite nanoparticles in Balb/3T3 mouse fibroblasts",

abstract = "The effect of the concentration of cobalt ferrite (CoFe 2O 4) nanoparticles (NPs) on their intracellular location and distribution has been explored by synchrotron radiation X-ray and fluorescence microscopy (SR-XRF) monitoring the evolution of NPs elemental composition as well. In cells exposed to low concentrations of CoFe 2O 4 NPs, the NPs preferentially segregate in the perinuclear region preserving their initial chemical content. At concentrations exceeding 500μM the XRF spectra indicate the presence of Co and Fe also in the nuclear region, accompanied by sensible changes in the cellular morphology. The increase of the Co/Fe ratio measured in the nuclear compartment indicates that above certain concentrations the CoFe 2O 4 NPs intracellular distribution could be accompanied by biodegradation resulting in Co accumulation in the nucleus.",

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AU - Ponti, Jessica

AU - Rossi, François

AU - Salomé, Murielle

AU - Kaulich, Burkhard

AU - Kiskinova, Maya

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