Cellular distribution of Hsp70 expression in rat skeletal muscles. Effects of moderate exercise training and chronic hypoxia

Elena Tarricone, Cristina Scapin, Maurizio Vitadello, Fabio Esposito, Vittoria Margonato, Giuseppina Milano, Michele Samaja, Luisa Gorza

Research output: Contribution to journalArticle

24 Citations (Scopus)

Abstract

Rat hindlimb muscles constitutively express the inducible heat shock protein 72 (Hsp70), apparently in proportion to the slow myosin content. Since it remains controversial whether chronic Hsp70 expression reflects the overimposed stress, we investigated Hsp70 cellular distribution in fast muscles of the posterior rat hindlimb after (1) mild exercise training (up to 30 m/min treadmill run for 1 h/day), which induces a remodeling in fast fiber composition, or (2) prolonged exposure to normobaric hypoxia (10%O2), which does not affect fiber-type composition. Both conditions increased significantly protein Hsp70 levels in the skeletal muscle. Immunohistochemistry showed the labeling for Hsp70 in subsets of both slow/ type 1 and fast/type 2A myofibers of control, sedentary, and normoxic rats. Endurance training increased about threefold the percentage of Hsp70-positive myofibers (P

Original languageEnglish
Pages (from-to)483-495
Number of pages13
JournalCell Stress and Chaperones
Volume13
Issue number4
DOIs
Publication statusPublished - Dec 2008

Fingerprint

Muscle
Rats
Skeletal Muscle
Exercise
Hindlimb
HSP72 Heat-Shock Proteins
Muscles
Exercise equipment
Fibers
Myosins
Chemical analysis
Labeling
Durability
Immunohistochemistry
Hypoxia
Proteins

Keywords

  • Exercise
  • HO-1
  • HSP70
  • Hypoxia
  • Skeletal muscle

ASJC Scopus subject areas

  • Biochemistry
  • Cell Biology

Cite this

Cellular distribution of Hsp70 expression in rat skeletal muscles. Effects of moderate exercise training and chronic hypoxia. / Tarricone, Elena; Scapin, Cristina; Vitadello, Maurizio; Esposito, Fabio; Margonato, Vittoria; Milano, Giuseppina; Samaja, Michele; Gorza, Luisa.

In: Cell Stress and Chaperones, Vol. 13, No. 4, 12.2008, p. 483-495.

Research output: Contribution to journalArticle

Tarricone, Elena ; Scapin, Cristina ; Vitadello, Maurizio ; Esposito, Fabio ; Margonato, Vittoria ; Milano, Giuseppina ; Samaja, Michele ; Gorza, Luisa. / Cellular distribution of Hsp70 expression in rat skeletal muscles. Effects of moderate exercise training and chronic hypoxia. In: Cell Stress and Chaperones. 2008 ; Vol. 13, No. 4. pp. 483-495.
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