Cellular endotoxin desensitization in patients with severe chronic heart failure

Rakesh Sharma, Aidan P. Bolger, Mathias Rauchhaus, Stephan Von Haehling, Wolfram Doehner, Ian M. Adcock, Peter J. Barnes, Philip A. Poole-Wilson, Hans Dieter Volk, Andrew J S Coats, Sam Lim, Stefan D. Anker

Research output: Contribution to journalArticle

Abstract

Background: Increased levels of bacterial lipopolysaccharide (LPS) have been demonstrated in chronic heart failure (CHF). LPS can induce cellular desensitization, with specific down-regulation of LPS-mediated cellular tumor necrosis factor (TNF-α) production which does not affect other cytokine parameters. It is not known if LPS desensitization occurs in CHF. Methods and results: Mononuclear cells from 24 CHF patients (mean age 70±2 years, age range 58 to 78 years, NYHA class 3.0±0.2) and 11 healthy controls (mean age 53±3 years, age range 39 to 75 years) were separated from venous blood and cultured for 24 h with LPS (E. coli, 0-10 ng/mL). Culture supernatants were tested for TNF-α and interleukin-1 receptor antagonist (IL-1RA). Patients were subgrouped into mild (n=10), moderate (n=5), and severe (n=9) CHF. Independently of age, mononuclear cells from patients with severe heart failure produced less TNF-α than controls (p

Original languageEnglish
Pages (from-to)865-868
Number of pages4
JournalEuropean Journal of Heart Failure
Volume7
Issue number5
DOIs
Publication statusPublished - Aug 2005

Keywords

  • Cytokines
  • Heart failure
  • Lipopolysaccharide

ASJC Scopus subject areas

  • Cardiology and Cardiovascular Medicine

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