Abstract
Background: Increased levels of bacterial lipopolysaccharide (LPS) have been demonstrated in chronic heart failure (CHF). LPS can induce cellular desensitization, with specific down-regulation of LPS-mediated cellular tumor necrosis factor (TNF-α) production which does not affect other cytokine parameters. It is not known if LPS desensitization occurs in CHF. Methods and results: Mononuclear cells from 24 CHF patients (mean age 70±2 years, age range 58 to 78 years, NYHA class 3.0±0.2) and 11 healthy controls (mean age 53±3 years, age range 39 to 75 years) were separated from venous blood and cultured for 24 h with LPS (E. coli, 0-10 ng/mL). Culture supernatants were tested for TNF-α and interleukin-1 receptor antagonist (IL-1RA). Patients were subgrouped into mild (n=10), moderate (n=5), and severe (n=9) CHF. Independently of age, mononuclear cells from patients with severe heart failure produced less TNF-α than controls (p
| Original language | English |
|---|---|
| Pages (from-to) | 865-868 |
| Number of pages | 4 |
| Journal | European Journal of Heart Failure |
| Volume | 7 |
| Issue number | 5 |
| DOIs | |
| Publication status | Published - Aug 2005 |
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Keywords
- Cytokines
- Heart failure
- Lipopolysaccharide
ASJC Scopus subject areas
- Cardiology and Cardiovascular Medicine
Cite this
Cellular endotoxin desensitization in patients with severe chronic heart failure. / Sharma, Rakesh; Bolger, Aidan P.; Rauchhaus, Mathias; Von Haehling, Stephan; Doehner, Wolfram; Adcock, Ian M.; Barnes, Peter J.; Poole-Wilson, Philip A.; Volk, Hans Dieter; Coats, Andrew J S; Lim, Sam; Anker, Stefan D.
In: European Journal of Heart Failure, Vol. 7, No. 5, 08.2005, p. 865-868.Research output: Contribution to journal › Article
}
TY - JOUR
T1 - Cellular endotoxin desensitization in patients with severe chronic heart failure
AU - Sharma, Rakesh
AU - Bolger, Aidan P.
AU - Rauchhaus, Mathias
AU - Von Haehling, Stephan
AU - Doehner, Wolfram
AU - Adcock, Ian M.
AU - Barnes, Peter J.
AU - Poole-Wilson, Philip A.
AU - Volk, Hans Dieter
AU - Coats, Andrew J S
AU - Lim, Sam
AU - Anker, Stefan D.
PY - 2005/8
Y1 - 2005/8
N2 - Background: Increased levels of bacterial lipopolysaccharide (LPS) have been demonstrated in chronic heart failure (CHF). LPS can induce cellular desensitization, with specific down-regulation of LPS-mediated cellular tumor necrosis factor (TNF-α) production which does not affect other cytokine parameters. It is not known if LPS desensitization occurs in CHF. Methods and results: Mononuclear cells from 24 CHF patients (mean age 70±2 years, age range 58 to 78 years, NYHA class 3.0±0.2) and 11 healthy controls (mean age 53±3 years, age range 39 to 75 years) were separated from venous blood and cultured for 24 h with LPS (E. coli, 0-10 ng/mL). Culture supernatants were tested for TNF-α and interleukin-1 receptor antagonist (IL-1RA). Patients were subgrouped into mild (n=10), moderate (n=5), and severe (n=9) CHF. Independently of age, mononuclear cells from patients with severe heart failure produced less TNF-α than controls (p
AB - Background: Increased levels of bacterial lipopolysaccharide (LPS) have been demonstrated in chronic heart failure (CHF). LPS can induce cellular desensitization, with specific down-regulation of LPS-mediated cellular tumor necrosis factor (TNF-α) production which does not affect other cytokine parameters. It is not known if LPS desensitization occurs in CHF. Methods and results: Mononuclear cells from 24 CHF patients (mean age 70±2 years, age range 58 to 78 years, NYHA class 3.0±0.2) and 11 healthy controls (mean age 53±3 years, age range 39 to 75 years) were separated from venous blood and cultured for 24 h with LPS (E. coli, 0-10 ng/mL). Culture supernatants were tested for TNF-α and interleukin-1 receptor antagonist (IL-1RA). Patients were subgrouped into mild (n=10), moderate (n=5), and severe (n=9) CHF. Independently of age, mononuclear cells from patients with severe heart failure produced less TNF-α than controls (p
KW - Cytokines
KW - Heart failure
KW - Lipopolysaccharide
UR - http://www.scopus.com/inward/record.url?scp=19544365810&partnerID=8YFLogxK
UR - http://www.scopus.com/inward/citedby.url?scp=19544365810&partnerID=8YFLogxK
U2 - 10.1016/j.ejheart.2004.09.014
DO - 10.1016/j.ejheart.2004.09.014
M3 - Article
C2 - 16087139
AN - SCOPUS:19544365810
VL - 7
SP - 865
EP - 868
JO - European Journal of Heart Failure
JF - European Journal of Heart Failure
SN - 1388-9842
IS - 5
ER -