Cellular microchimerism as a lifelong physiologic status in parous women: An immunologic basis for its amplification in patients with systemic sclerosis

Samuele E. Burastero, Silvia Galbiati, Antonia Vassallo, Maria Grazia Sabbadini, Matteo Bellone, Lucia Marchionni, Maddalena Smid, Elisabetta Ferrero, Augusto Ferrari, Maurizio Ferrari, Laura Cremonesi

Research output: Contribution to journalArticle

Abstract

Objective. To quantitatively measure male DNA in blood from women with systemic sclerosis (SSc) and from controls and to evaluate in vitro the modulation of the microchimeric cell population size following immunologic stimuli that were expected to trigger antigen-specific T cells. Methods. A real-time polymerase chain reaction for a Y chromosome sequence was used to measure male DNA in blood from women with SSc and from controls who gave birth to sons. The in vitro change in the microchimeric cell population size was measured following immunologic stimuli, which were expected to trigger antigen-specific T cells. Results. Cellular microchimerism was found in SSc patients and controls, but the absolute amount of male DNA was higher in the patients, and the in vitro addition to blood mononuclear cells of an anti-CD28 costimulatory signal acted as a powerful amplification of microchimeric cells in 3 patients with SSc but not in controls. Conclusion. Cellular microchimerism is a physiologic phenomenon in parous women. In SSc patients, cellular microchimerism is accounted for by a higher number of cells that have the characteristics of T lymphocytes specific to maternal allogeneic antigens.

Original languageEnglish
Pages (from-to)1109-1116
Number of pages8
JournalArthritis and Rheumatism
Volume48
Issue number4
DOIs
Publication statusPublished - Apr 1 2003

ASJC Scopus subject areas

  • Immunology
  • Rheumatology

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