Cellular uptake and delivery monitoring of liposome/DNA complexes during in vitro transfection of CFTR gene

Anna Lucia Serafino; Giuseppe Novelli; Sabrina Di Sario; Alessia Colosimo; Paola Amicucci; Federica Sangiuolo; Giuseppe Mossa; Bruno Dallapiccola

Cellular uptake and delivery monitoring of liposome/DNA complexes during in vitro transfection of CFTR gene

Anna Lucia Serafino, Giuseppe Novelli, Sabrina Di Sario, Alessia Colosimo, Paola Amicucci, Federica Sangiuolo, Giuseppe Mossa, Bruno Dallapiccola

Research output: Contribution to journal › Article › peer-review

Abstract

The cellular uptake and distribution of cationic liposomes Dc-Chol/DOPE-CFTR gene complexes were assessed by electronic and confocal laser scanner microscopy (CLSM) for the CFTR gene transfer to human adenocarcinoma and tracheal epithelial cell lines. Cationic lipid forms unilamellar and multilamellar vesicles capable of rapid and efficient transport of gene into target cells. The number of fluorescent complexes was increasing with time in cells up to 6 hours showing a punctate and homogeneous DNA distribution in the cytoplasmatic and nuclear compartments, including the nucleolus. No significant difference in the biochemical and cellular behavior was observed between the investigated system and other systems previously tested. This study adds new insights into the CFTR cationic liposome-mediated gene delivery.

Original language	English
Pages (from-to)	337-344
Number of pages	8
Journal	Biochemistry and Molecular Biology International
Volume	47
Issue number	2
Publication status	Published - Feb 1999

Keywords

Cationic liposomes
Confocal laser scanning microscopy
Cystic fibrosis
Gene therapy

ASJC Scopus subject areas

Biochemistry
Genetics
Molecular Biology

Fingerprint Dive into the research topics of 'Cellular uptake and delivery monitoring of liposome/DNA complexes during in vitro transfection of CFTR gene'. Together they form a unique fingerprint.

Cite this

Cellular uptake and delivery monitoring of liposome/DNA complexes during in vitro transfection of CFTR gene. / Serafino, Anna Lucia; Novelli, Giuseppe; Di Sario, Sabrina; Colosimo, Alessia; Amicucci, Paola; Sangiuolo, Federica; Mossa, Giuseppe; Dallapiccola, Bruno.

In: Biochemistry and Molecular Biology International, Vol. 47, No. 2, 02.1999, p. 337-344.

Research output: Contribution to journal › Article › peer-review

@article{964d131fcff043cbb06a8d46720be011,

title = "Cellular uptake and delivery monitoring of liposome/DNA complexes during in vitro transfection of CFTR gene",

abstract = "The cellular uptake and distribution of cationic liposomes Dc-Chol/DOPE-CFTR gene complexes were assessed by electronic and confocal laser scanner microscopy (CLSM) for the CFTR gene transfer to human adenocarcinoma and tracheal epithelial cell lines. Cationic lipid forms unilamellar and multilamellar vesicles capable of rapid and efficient transport of gene into target cells. The number of fluorescent complexes was increasing with time in cells up to 6 hours showing a punctate and homogeneous DNA distribution in the cytoplasmatic and nuclear compartments, including the nucleolus. No significant difference in the biochemical and cellular behavior was observed between the investigated system and other systems previously tested. This study adds new insights into the CFTR cationic liposome-mediated gene delivery.",

keywords = "Cationic liposomes, Confocal laser scanning microscopy, Cystic fibrosis, Gene therapy",

author = "Serafino, {Anna Lucia} and Giuseppe Novelli and {Di Sario}, Sabrina and Alessia Colosimo and Paola Amicucci and Federica Sangiuolo and Giuseppe Mossa and Bruno Dallapiccola",

year = "1999",

month = feb,

language = "English",

volume = "47",

pages = "337--344",

journal = "Biochemistry and Molecular Biology International",

issn = "1039-9712",

publisher = "Academic Press Inc.",

number = "2",

}

TY - JOUR

T1 - Cellular uptake and delivery monitoring of liposome/DNA complexes during in vitro transfection of CFTR gene

AU - Serafino, Anna Lucia

AU - Novelli, Giuseppe

AU - Di Sario, Sabrina

AU - Colosimo, Alessia

AU - Amicucci, Paola

AU - Sangiuolo, Federica

AU - Mossa, Giuseppe

AU - Dallapiccola, Bruno

PY - 1999/2

Y1 - 1999/2

N2 - The cellular uptake and distribution of cationic liposomes Dc-Chol/DOPE-CFTR gene complexes were assessed by electronic and confocal laser scanner microscopy (CLSM) for the CFTR gene transfer to human adenocarcinoma and tracheal epithelial cell lines. Cationic lipid forms unilamellar and multilamellar vesicles capable of rapid and efficient transport of gene into target cells. The number of fluorescent complexes was increasing with time in cells up to 6 hours showing a punctate and homogeneous DNA distribution in the cytoplasmatic and nuclear compartments, including the nucleolus. No significant difference in the biochemical and cellular behavior was observed between the investigated system and other systems previously tested. This study adds new insights into the CFTR cationic liposome-mediated gene delivery.

AB - The cellular uptake and distribution of cationic liposomes Dc-Chol/DOPE-CFTR gene complexes were assessed by electronic and confocal laser scanner microscopy (CLSM) for the CFTR gene transfer to human adenocarcinoma and tracheal epithelial cell lines. Cationic lipid forms unilamellar and multilamellar vesicles capable of rapid and efficient transport of gene into target cells. The number of fluorescent complexes was increasing with time in cells up to 6 hours showing a punctate and homogeneous DNA distribution in the cytoplasmatic and nuclear compartments, including the nucleolus. No significant difference in the biochemical and cellular behavior was observed between the investigated system and other systems previously tested. This study adds new insights into the CFTR cationic liposome-mediated gene delivery.

KW - Cationic liposomes

KW - Confocal laser scanning microscopy

KW - Cystic fibrosis

KW - Gene therapy

UR - http://www.scopus.com/inward/record.url?scp=0032986952&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=0032986952&partnerID=8YFLogxK

M3 - Article

C2 - 10205680

AN - SCOPUS:0032986952

VL - 47

SP - 337

EP - 344

JO - Biochemistry and Molecular Biology International

JF - Biochemistry and Molecular Biology International

SN - 1039-9712

IS - 2

ER -