Vitalità cellulare e risposta immunologica negli omoinnesti valvolari cardiaci criopreservati: Una revisione critica

Translated title of the contribution: Cellular viability and immunology in cryopreserved homograft valves. A critical review

G. Pompilio, G. L. Polvani, M. Porqueddu, A. Guarino, A. Sala, P. Biglioli

Research output: Contribution to journalArticle

Abstract

Background. Long-term durability of homograft valves is related to cellular viability and immunological compatibility. In this paper, we carry out a critical update of the relationship between cellular viability and immunology of cryopreserved homograft valves. Biological issue. In the first section, we review the theoretical background of the superior durability of cryopreserved valves with high cellular viability, with respect to the "fresh" antibiotic-stored valves, with lower viability rates. Afterwards, a brief review of the principal factors influencing the homograft valve viability rate during harvesting, preparation, sterilization and cryopreservation phases, is performed. Cellular viability. In the second paragraph, we analyze the problem of cellular viability in cryopreserved valves, both for matrix cells - fibroblast - and for endothelium. In particular, we report the current methods of quantifications of viability and more recent biological researches. Correlations between biological and clinical data are also discussed. Immunological issue. Finally, the immunological issue and its relationships with cellular viability is analyzed, with particular regard to experimental evidence and clinical implications. Moreover, we have described the recent hypothesis on the influence of cryopreservation on the antigenicity of tissues, and the laboratory researches on the long-term antibody response in humans after homograft valve implantation.

Translated title of the contributionCellular viability and immunology in cryopreserved homograft valves. A critical review
Original languageItalian
Pages (from-to)235-244
Number of pages10
JournalMinerva Cardioangiologica
Volume45
Issue number5
Publication statusPublished - May 1997

ASJC Scopus subject areas

  • Cardiology and Cardiovascular Medicine

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