Centrosomal and mitotic abnormalities in cell lines derived from papillary thyroid cancer harboring specific gene alterations

Irena Maric, Silvia Viaggi, Paola Caria, Daniela V. Frau, Paolo Degan, Roberta Vanni

Research output: Contribution to journalArticle

Abstract

Background: Differentiated thyroid carcinoma offers a good model to investigate the possible correlation between specific gene mutations and chromosome instability. Papillary thyroid neoplasms are characterized by different mutually exclusive genetic alterations, some of which are associated with aneuploidy and aggressive phenotype. Results: We investigated the centrosome status and mitotic abnormalities in three thyroid carcinoma-derived cell lines, each maintaining the specific, biologically relevant gene alteration harbored by the parental tumors: RET/PTC1 rearrangement in TPC1; heterozygous and homozygous BRAF V600E mutation in K1 and in B-CPAP, respectively. B-CPAP cells showed a statistically significant (P <0.01) higher frequency of abnormal mitotic figures compared to TPC1 and K1 cells. Conclusions: Our data indicate that RET/PTC1 oncogenic activity is not related to mitotic chromosome impairment and missegregation whereas, based on the consistent difference in types/frequencies of centrosome and spindle abnormalities observed between K1 and B-CPAP cells, the hetero/homozygous allelic status of BRAF V600E mutation seems to be not irrelevant in respect to chromosomal instability development.

Original languageEnglish
Article number26
JournalMolecular Cytogenetics
Volume4
Issue number1
DOIs
Publication statusPublished - 2011

Keywords

  • BRAF
  • centrosome
  • mitotic spindle
  • RET/PTC
  • thyroid carcinoma

ASJC Scopus subject areas

  • Genetics
  • Molecular Biology
  • Genetics(clinical)
  • Biochemistry
  • Molecular Medicine
  • Biochemistry, medical

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