Ceramide composition of the psoriatic scale

Stefania Motta, Marcello Monti, Silvia Sesana, Ruggero Caputo, Stephana Carelli, Riccardo Ghidoni

Research output: Contribution to journalArticle

Abstract

This paper investigates the ceramide composition of the psoriatic scale compared with that of normal human SC. A method was optimalized, based on TLC separation followed by densitometry, allowing the provision of good resolution and quantification of ceramide fractions from both normal and pathological specimens. Seven ceramide fractions were isolated and submitted to compositional analysis. The obtained results suggested a revisitation of previous ceramide designation. Therefore a simple classification is suggested, based on grouping ceramides carrying structural similarities under common codes. According to these rules, ceramides were grouped into five classes designated as: (1) Cer[EOS], which contains ester-linked fatty acids, ω-OH fatty acids and sphingosines; (2) Cer[NS], which contains non-OH fatty acids and sphingosines; (3) Cer[NP], which contains non-OH fatty acids and phytosphingosines; (4) Cer[AS], which contains α-OH fatty acids and sphingosines; (5) Cer[AP], which contains α-OH fatty acids and phytosphingosines. Analysis of ceramides from the psoriatic scale, compared to those from normal human SC, resulted in an impairment of the Cer[EOS] content as well as of the ceramides containing phytosphingosine, with concurrent increase in ceramides containing sphingosine, being the total amount maintained identical. Since one of the suggested pathways for phytosphingosine biosynthesis involves the water addition to the corresponding sphingosine double bond, we can speculate that the observed alterarion is due to a deranged water bioavailability, associated with psoriaris.

Original languageEnglish
Pages (from-to)147-151
Number of pages5
JournalBiochimica et Biophysica Acta - Molecular Basis of Disease
Volume1182
Issue number2
DOIs
Publication statusPublished - Sep 8 1993

Keywords

  • Phytosphingosine
  • Psoriasis
  • Skin
  • Sphingosine
  • Stratum corneum

ASJC Scopus subject areas

  • Biophysics
  • Molecular Biology
  • Molecular Medicine

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  • Cite this

    Motta, S., Monti, M., Sesana, S., Caputo, R., Carelli, S., & Ghidoni, R. (1993). Ceramide composition of the psoriatic scale. Biochimica et Biophysica Acta - Molecular Basis of Disease, 1182(2), 147-151. https://doi.org/10.1016/0925-4439(93)90135-N