Ceramide inhibits antigen uptake and presentation by dendritic cells

Federica Sallusto, Chiara Nicolò, Ruggero De Maria, Silvia Corinti, Roberto Testi

Research output: Contribution to journalArticlepeer-review

Abstract

Ceramides are intramembrane diffusible mediators involved in transducing signals originated from a variety of cell surface receptors. Different adaptive and differentiative cellular responses, including apoptotic cell death, use ceramide-mediated pathways as an essential part of the program. Here, we show that human dendritic cells respond to CD40 ligand, as well as to tumor necrosis factor-α and IL-1β, with intracellular ceramide accumulation, as they are induced to differentiate. Dendritic cells down- modulate their capacity to take up soluble antigens in response to exogenously added or endogenously produced ceramides. This is followed by an impairment in presenting soluble antigens to specific T cell clones, while cell viability and the capacity to stimulate allogeneic responses or to present immunogenic peptides is fully preserved. Thus, ceramide-mediated pathways initiated by different cytokines can actively modulate professional antigen-presenting cell function and antigen-specific immune responses.

Original languageEnglish
Pages (from-to)2411-2416
Number of pages6
JournalJournal of Experimental Medicine
Volume184
Issue number6
DOIs
Publication statusPublished - Dec 1 1996

ASJC Scopus subject areas

  • Immunology

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