Cerato-platanin, the first member of a new fungal protein family: Cloning, expression, and characterization

Luigia Pazzagli, Barbara Pantera, Lara Carresi, Camilla Zoppi, Thelma A. Pertinhez, Alberto Spisni, Stefania Tegli, Aniello Scala, Gianni Cappugi

Research output: Contribution to journalArticle

Abstract

The ascomycete Ceratocystis fimbriata, the causal agent of "canker stain disease," secretes a protein of 12.4 kDa that elicits phytoalexin synthesis and plant cell death. This protein, named cerato-platanin (CP), is also located in the cell walls of ascospores, hyphae, and conidia; it contains four cysteines (S-S bridged) and is moderately hydrophobic. The cp gene consists of a single exon and has 42 bp codifying for a signal peptide of 14 residues. The recombinant protein was obtained by cloning the cp gene of the mature protein in Escherichia coli (BL21), and a refolding step was needed to achieve the native active form. In the European Molecular Biology data bank, CP is reported as the first member of the CP family; this is the first example of an set of secreted fungal proteins whose primary structure is very similar. Nonetheless, the data also revealed some structural and functional features that make CP similar to proteins of the hydrophobin family.

Original languageEnglish
Pages (from-to)512-521
Number of pages10
JournalCell Biochemistry and Biophysics
Volume44
Issue number3
DOIs
Publication statusPublished - 2006

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Keywords

  • Cerato-platanin family
  • Cloning
  • Eliciting activity
  • Hydrophobin
  • Protein expression
  • Refolding

ASJC Scopus subject areas

  • Cell Biology
  • Clinical Biochemistry
  • Biophysics
  • Biochemistry

Cite this

Pazzagli, L., Pantera, B., Carresi, L., Zoppi, C., Pertinhez, T. A., Spisni, A., Tegli, S., Scala, A., & Cappugi, G. (2006). Cerato-platanin, the first member of a new fungal protein family: Cloning, expression, and characterization. Cell Biochemistry and Biophysics, 44(3), 512-521. https://doi.org/10.1385/CBB:44:3:512