TY - JOUR
T1 - Cerato-platanin, the first member of a new fungal protein family
T2 - Cloning, expression, and characterization
AU - Pazzagli, Luigia
AU - Pantera, Barbara
AU - Carresi, Lara
AU - Zoppi, Camilla
AU - Pertinhez, Thelma A.
AU - Spisni, Alberto
AU - Tegli, Stefania
AU - Scala, Aniello
AU - Cappugi, Gianni
PY - 2006
Y1 - 2006
N2 - The ascomycete Ceratocystis fimbriata, the causal agent of "canker stain disease," secretes a protein of 12.4 kDa that elicits phytoalexin synthesis and plant cell death. This protein, named cerato-platanin (CP), is also located in the cell walls of ascospores, hyphae, and conidia; it contains four cysteines (S-S bridged) and is moderately hydrophobic. The cp gene consists of a single exon and has 42 bp codifying for a signal peptide of 14 residues. The recombinant protein was obtained by cloning the cp gene of the mature protein in Escherichia coli (BL21), and a refolding step was needed to achieve the native active form. In the European Molecular Biology data bank, CP is reported as the first member of the CP family; this is the first example of an set of secreted fungal proteins whose primary structure is very similar. Nonetheless, the data also revealed some structural and functional features that make CP similar to proteins of the hydrophobin family.
AB - The ascomycete Ceratocystis fimbriata, the causal agent of "canker stain disease," secretes a protein of 12.4 kDa that elicits phytoalexin synthesis and plant cell death. This protein, named cerato-platanin (CP), is also located in the cell walls of ascospores, hyphae, and conidia; it contains four cysteines (S-S bridged) and is moderately hydrophobic. The cp gene consists of a single exon and has 42 bp codifying for a signal peptide of 14 residues. The recombinant protein was obtained by cloning the cp gene of the mature protein in Escherichia coli (BL21), and a refolding step was needed to achieve the native active form. In the European Molecular Biology data bank, CP is reported as the first member of the CP family; this is the first example of an set of secreted fungal proteins whose primary structure is very similar. Nonetheless, the data also revealed some structural and functional features that make CP similar to proteins of the hydrophobin family.
KW - Cerato-platanin family
KW - Cloning
KW - Eliciting activity
KW - Hydrophobin
KW - Protein expression
KW - Refolding
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U2 - 10.1385/CBB:44:3:512
DO - 10.1385/CBB:44:3:512
M3 - Article
C2 - 16679539
AN - SCOPUS:33646855776
VL - 44
SP - 512
EP - 521
JO - Cell Biochemistry and Biophysics
JF - Cell Biochemistry and Biophysics
SN - 1085-9195
IS - 3
ER -