Cerebellar neurons and glial cells are transducible by lentiviral vectors without decrease of cerebellar functions

C. Croci, S. Fasano, D. Superchi, L. Perani, A. Martellosio, R. Brambilla, G. Consalez, E. R. Bongarzone

Research output: Contribution to journalArticle

Abstract

Due to the profuse connections of the cerebellum to the rest of the central nervous system, cerebellar dysfunction impacts tremendously on movement coordination, maintenance of equilibrium, muscle tone and motor memory. Efficient gene transfer of therapeutic genes to this central nervous system structure would constitute a relevant step ahead the design of treatments to ameliorate cerebellar dysfunction. Lentiviral vectors (LVs) have been used as efficient vehicles to integrate transgenes into dividing and non-dividing cells, such as postmitotic adult neurons, with minimal toxicity and immune response. This study aimed to use LVs carrying green fluorescent protein (GFP) cDNA for transduction of cerebellar cells in vivo without compromising neurological cerebellar functions. Our results indicate that LVs, injected in the lobulus simplex, transduced different cerebellar neurons including stellate, Purkinje cells, granular neurons and glial cells such as astrocytes, oligodendrocytes, and that this gene transfer approach was not accompanied by cerebellar deficits.

Original languageEnglish
Pages (from-to)216-221
Number of pages6
JournalDevelopmental Neuroscience
Volume28
Issue number3
DOIs
Publication statusPublished - May 2006

Keywords

  • Cerebellum
  • Gene therapy
  • Granule neurons
  • Lentiviral vector
  • Oligodendrocytes
  • Purkinje cells

ASJC Scopus subject areas

  • Neuroscience(all)

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    Croci, C., Fasano, S., Superchi, D., Perani, L., Martellosio, A., Brambilla, R., Consalez, G., & Bongarzone, E. R. (2006). Cerebellar neurons and glial cells are transducible by lentiviral vectors without decrease of cerebellar functions. Developmental Neuroscience, 28(3), 216-221. https://doi.org/10.1159/000091919