Cerebrospinal fluid analysis in HIV-1-infected children: Immunological and virological findings before and after AZT therapy

A. M. Laverda, P. Gallo, A. De Rossi, S. Sivieri, P. Cogo, A. Pagliaro, L. Chieco-Bianchi, B. Tavolato

Research output: Contribution to journalArticlepeer-review

Abstract

Immunological and viral studies were conducted on cerebrospinal fluid from 31 HIV-1-infected children, of whom 23 were neurologically asymptomatic and 8 had progressive encephalopathy. After AZT treatment, a second cerebrospinal fluid specimen was obtained from 15 children, 11 of whom were neurologically asymptomatic and 4 had progressive encephalopathy. Virus isolation and p24Ag detection were more frequent in children with progressive encephalopathy than in asymptomatic children (66% versus 12%) and were inversely correlated with intrathecal HIV-1-antibody detection (anti-gag AB: 25% versus 70%). High concentrations of interleukin-1β (IL-1β) and IL-6 were found in children with progressive encephalopathy (50% and 37%, respectively), but low levels were also detected in some asymptomatic children (13% and 9%, respectively). Tumour necrosis factor-α (TNFα) was not found. AZT treatment induced disappearance of p24Ag in cerebrospinal fluid, as well as a marked reduction in cytokine levels. Cytokine determination may be useful in monitoring AZT treatment in children with progressive encephalopathy.

Original languageEnglish
Pages (from-to)1038-1042
Number of pages5
JournalActa Paediatrica, International Journal of Paediatrics
Volume83
Issue number10
Publication statusPublished - 1994

Keywords

  • AZT
  • Cerebrospinal fluid
  • Children
  • Cytokines
  • HIV-1

ASJC Scopus subject areas

  • Pediatrics, Perinatology, and Child Health

Fingerprint

Dive into the research topics of 'Cerebrospinal fluid analysis in HIV-1-infected children: Immunological and virological findings before and after AZT therapy'. Together they form a unique fingerprint.

Cite this