Cerebrospinal fluid in the diagnosis of multiple sclerosis: A consensus report

M. Andersson, J. Alvarez-Cermeñio, G. Bernardi, I. Cogato, P. Fredman, J. Frederiksen, S. Fredrikson, P. Gallo, L. M. Grimaldi, M. Grønning, G. Keir, K. Lamers, H. Link, A. Magalhães, A. R. Massaro, S. Öhman, H. Reiber, L. Rönnbäck, M. Schluep, E. SchullerC. J M Sindic, E. J. Thompson, M. Trojano, U. Wurster

Research output: Contribution to journalArticlepeer-review


The Committee of the European Concerted Action for Multiple Sclerosis (Charcot Foundation) organised five workshops to discuss CSF analytical standards in the diagnosis of multiple sclerosis. This consensus report from 12 European countries summarises the results of those workshops. It is hoped that neurologists will confer with their colleagues in clinical chemistry to arrange the best possible local practice. The most sensitive method for the detection of oligoclonal immunoglobulin bands is isoelectric focusing. The same amounts of IgG in parallel CSF and serum samples are used and oligoclonal bands are revealed with IgG specific antibody staining. All laboratories performing isoelectric focusing should check their technique at least annually using "blind" standards for the five different CSF and serum patterns. Quantitative measurements of IgG production in the CNS are less sensitive than isoelectric focusing. The preferred method for detection of blood-CSF barrier dysfunction is the albumin quotient. The CSF albumin or total protein concentrations are less satisfactory. These results must be interpreted with reference to the age of the patient and the local method of determination. Cells should be counted. The normal value is no more than 4 cells/μl. Among evolving optional tests, measurement of the combined local synthesis of antibodies against measles, rubella, and/or varicella zoster could represent a significant advance if it offers higher specificity (not sensitivity) for identifying chronic rather than acute inflammation. Other tests that may have useful correlations with clinical indices include those for oligoclonal free light chains, IgM, IgA, or myelin basic protein concentrations.

Original languageEnglish
Pages (from-to)897-902
Number of pages6
JournalJournal of Neurology, Neurosurgery and Psychiatry
Issue number8
Publication statusPublished - 1994

ASJC Scopus subject areas

  • Public Health, Environmental and Occupational Health
  • Neuropsychology and Physiological Psychology
  • Neuroscience(all)
  • Psychiatry and Mental health


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