Cerebrospinal fluid levels of the endocannabinoid anandamide are reduced in patients with untreated newly diagnosed temporal lobe epilepsy

Andrea Romigi, Monica Bari, Fabio Placidi, M. Grazia Marciani, Marilena Malaponti, Federico Torelli, Francesca Izzi, Chiara Prosperetti, Silvana Zannino, Francesca Corte, Carlo Chiaramonte, Mauro Maccarrone

Research output: Contribution to journalArticle

Abstract

Purpose: The endocannabinoid system is involved in excitatory/inhibitory balance mechanisms within the central nervous system (CNS). Growing evidence shows that its perturbation leads to development of epileptic seizures in experimental models, thus indicating that endocannabinoids play an intrinsic protective role in suppressing pathologic neuronal excitability. Experimental data also demonstrate that the endocannabinoid anandamide (AEA) can antagonize epileptic discharges in hippocampal tissue. The objective of our study was to measure endocannabinoids levels in the cerebrospinal fluid (CSF) of drug-naive patients affected by temporal lobe epilepsy (TLE). Methods: We measured the levels of both AEA and the other endocannabinoid, 2-arachidonoylglycerol (2-AG), in the CSF of drug-naive patients with TLE. Results: A significant reduction of AEA was found in the CSF of patients with compared with healthy controls (epileptic patients = 2.55 ± 1.78 pmol/ml; healthy controls = 11.65 ± 7.53 pmol/ml; n = 9 for both groups, p <0.01). 2-AG levels, however, were not affected (epileptic patients = 209.5 ± 146.56; healthy controls = 159.6 ± 110.2) (n = 6 for both groups, p = 0.48). Discussion: Our findings seem to be consistent with experimental evidence demonstrating a significant prevention of epileptic seizures induced by endocannabinoids in models of epilepsy. Furthermore, they support the hypothesis that AEA may be involved in its pathogenesis, suggesting a hypothetical primary impairment of the endocannabinoid system in untreated TLE. The actual role of this in vivo dysregulation still remains unclear.

Original languageEnglish
Pages (from-to)768-772
Number of pages5
JournalEpilepsia
Volume51
Issue number5
DOIs
Publication statusPublished - May 2010

Keywords

  • 2-arachidonoylglycerol
  • Anandamide
  • Endocannabinoids
  • Temporal lobe epilepsy

ASJC Scopus subject areas

  • Clinical Neurology
  • Neurology

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    Romigi, A., Bari, M., Placidi, F., Grazia Marciani, M., Malaponti, M., Torelli, F., Izzi, F., Prosperetti, C., Zannino, S., Corte, F., Chiaramonte, C., & Maccarrone, M. (2010). Cerebrospinal fluid levels of the endocannabinoid anandamide are reduced in patients with untreated newly diagnosed temporal lobe epilepsy. Epilepsia, 51(5), 768-772. https://doi.org/10.1111/j.1528-1167.2009.02334.x