Cervical human papilloma virus (HPV) DNA primary screening test: Results of a population-based screening programme in central Italy

Basilio Passamonti, Daniela Gustinucci, Paolo Giorgi Rossi, Elena Cesarini, Simonetta Bulletti, Angela Carlani, Nadia Martinelli, Massimo Broccolini, Valentina D’Angelo, Maria Rosaria D’Amico, Eugenio Di Dato, Paola Galeazzi, Morena Malaspina, Nicoletta Spita, Beatrice Tintori, Maria Donata Giaimo

Research output: Contribution to journalArticle

1 Citation (Scopus)

Abstract

Objective: To present the results of the first and second round human papilloma virus (HPV)-based screening programme in the Umbria region after three years. Methods: From August 2010 to November 2011, the entire female population aged 35-64 in a local health district was invited for HPV testing (HPV-DNA cobas4800 on a liquid-based cytology sample). HPV-negative women were re-invited after three years. For HPV-positive women, a slide was prepared and interpreted. Positive cytologies were referred to colposcopy; negatives were referred to repeat HPV after one year. If HPV was persistently positive, women were referred to colposcopy; if negative, to normal screening. Indicators of the first and second round are compared with those of cytology screening in the same area in the preceding three years. Results: Participation was 56.5%, the same as cytology (56.6%). HPV-positivity was 6.4% (396/6272), cytology triage positivity was 35.6%; 251 cytology negative women were referred to one-year HPV retesting, 84.1% complied, and 55.5% were positive. Total colposcopy referral was 4.1%, and for cytology 1%. The detection rate for cervical intraepithelial neoplasia grade 2 or more severe was 10%, compared with 3.7% using cytology. After three years, HPV-positivity was 3.4% (129/3831), overall colposcopy referral was 2.3% (most at one-year follow-up), and detection rate was 0.5/1000. Conclusions: The first round detection rate was more than twice that of cytology screening, while colposcopy referral increased fourfold. At the second round, the detection rate decreased dramatically, showing that longer interval and more conservative protocols are needed.

Original languageEnglish
Pages (from-to)153-162
Number of pages10
JournalJournal of Medical Screening
Volume24
Issue number3
DOIs
Publication statusPublished - 2017

Fingerprint

Papillomaviridae
Italy
Cell Biology
Colposcopy
DNA
Population
Referral and Consultation
Cervical Intraepithelial Neoplasia
Triage

Keywords

  • Cervical cancer
  • Human papilloma virus
  • Screening

ASJC Scopus subject areas

  • Health Policy
  • Public Health, Environmental and Occupational Health

Cite this

Cervical human papilloma virus (HPV) DNA primary screening test : Results of a population-based screening programme in central Italy. / Passamonti, Basilio; Gustinucci, Daniela; Giorgi Rossi, Paolo; Cesarini, Elena; Bulletti, Simonetta; Carlani, Angela; Martinelli, Nadia; Broccolini, Massimo; D’Angelo, Valentina; D’Amico, Maria Rosaria; Di Dato, Eugenio; Galeazzi, Paola; Malaspina, Morena; Spita, Nicoletta; Tintori, Beatrice; Giaimo, Maria Donata.

In: Journal of Medical Screening, Vol. 24, No. 3, 2017, p. 153-162.

Research output: Contribution to journalArticle

Passamonti, B, Gustinucci, D, Giorgi Rossi, P, Cesarini, E, Bulletti, S, Carlani, A, Martinelli, N, Broccolini, M, D’Angelo, V, D’Amico, MR, Di Dato, E, Galeazzi, P, Malaspina, M, Spita, N, Tintori, B & Giaimo, MD 2017, 'Cervical human papilloma virus (HPV) DNA primary screening test: Results of a population-based screening programme in central Italy', Journal of Medical Screening, vol. 24, no. 3, pp. 153-162. https://doi.org/10.1177/0969141316663580
Passamonti, Basilio ; Gustinucci, Daniela ; Giorgi Rossi, Paolo ; Cesarini, Elena ; Bulletti, Simonetta ; Carlani, Angela ; Martinelli, Nadia ; Broccolini, Massimo ; D’Angelo, Valentina ; D’Amico, Maria Rosaria ; Di Dato, Eugenio ; Galeazzi, Paola ; Malaspina, Morena ; Spita, Nicoletta ; Tintori, Beatrice ; Giaimo, Maria Donata. / Cervical human papilloma virus (HPV) DNA primary screening test : Results of a population-based screening programme in central Italy. In: Journal of Medical Screening. 2017 ; Vol. 24, No. 3. pp. 153-162.
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abstract = "Objective: To present the results of the first and second round human papilloma virus (HPV)-based screening programme in the Umbria region after three years. Methods: From August 2010 to November 2011, the entire female population aged 35-64 in a local health district was invited for HPV testing (HPV-DNA cobas4800 on a liquid-based cytology sample). HPV-negative women were re-invited after three years. For HPV-positive women, a slide was prepared and interpreted. Positive cytologies were referred to colposcopy; negatives were referred to repeat HPV after one year. If HPV was persistently positive, women were referred to colposcopy; if negative, to normal screening. Indicators of the first and second round are compared with those of cytology screening in the same area in the preceding three years. Results: Participation was 56.5{\%}, the same as cytology (56.6{\%}). HPV-positivity was 6.4{\%} (396/6272), cytology triage positivity was 35.6{\%}; 251 cytology negative women were referred to one-year HPV retesting, 84.1{\%} complied, and 55.5{\%} were positive. Total colposcopy referral was 4.1{\%}, and for cytology 1{\%}. The detection rate for cervical intraepithelial neoplasia grade 2 or more severe was 10{\%}, compared with 3.7{\%} using cytology. After three years, HPV-positivity was 3.4{\%} (129/3831), overall colposcopy referral was 2.3{\%} (most at one-year follow-up), and detection rate was 0.5/1000. Conclusions: The first round detection rate was more than twice that of cytology screening, while colposcopy referral increased fourfold. At the second round, the detection rate decreased dramatically, showing that longer interval and more conservative protocols are needed.",
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AU - Cesarini, Elena

AU - Bulletti, Simonetta

AU - Carlani, Angela

AU - Martinelli, Nadia

AU - Broccolini, Massimo

AU - D’Angelo, Valentina

AU - D’Amico, Maria Rosaria

AU - Di Dato, Eugenio

AU - Galeazzi, Paola

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