Cfi 1 prevents premature exit from mitosis by anchoring Cdc14 phosphatase in the nucleolus

Rosella Visintin, Ellen S. Hwang, Angelika Amon

Research output: Contribution to journalArticle

451 Citations (Scopus)

Abstract

In eukaryotes, the activation of mitotic cyclin-dependent kinases (CDKs) induces mitosis, and their inactivation causes cells to leave mitosis. In budding yeast, two redundant mechanisms induce the inactivation of mitotic CDKs. In one mechanism, a specialized ubiquitin-dependent proteolytic system (called the APC-dependent proteolysis machinery) degrades the mitotic (Clb) cyclin subunit. In the other, the kinase-inhibitor Sic1 binds to mitotic CDKs and inhibits their kinase activity. The highly conserved protein phosphatase Cdc14 promotes both Clb degradation and Sic1 accumulation. Cdc14 promotes SIC1 transcription and the stabilization of Sic1 protein by dephosphorylating Sic1 and its transcription factor Swi5. Cdc14 activates the degradation of Clb cyclins by dephosphorylating the APC-specificity factor Cdh1 (refs 3, 4). So how is Cdc14 regulated? Here we show that Cdc14 is sequestered in the nucleolus for most of the cell cycle. During nuclear division, Cdc14 is released from the nucleolus, allowing it to reach its targets. A highly conserved signalling cascade, critical for the exit from mitosis, is required for this movement of Cdc14 during anaphase. Furthermore, we have identified a negative regulator of Cdc14, Cfi1, that anchors Cdc14 in the nucleolus.

Original languageEnglish
Pages (from-to)818-823
Number of pages6
JournalNature
Volume398
Issue number6730
DOIs
Publication statusPublished - Apr 29 1999

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Cyclin-Dependent Kinases
Phosphoric Monoester Hydrolases
Mitosis
Cyclins
Phosphotransferases
Cell Nucleus Division
Anaphase
Saccharomycetales
Phosphoprotein Phosphatases
Ubiquitin
Eukaryota
Proteolysis
Cell Cycle
Transcription Factors
Proteins

ASJC Scopus subject areas

  • General

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Cfi 1 prevents premature exit from mitosis by anchoring Cdc14 phosphatase in the nucleolus. / Visintin, Rosella; Hwang, Ellen S.; Amon, Angelika.

In: Nature, Vol. 398, No. 6730, 29.04.1999, p. 818-823.

Research output: Contribution to journalArticle

Visintin, Rosella ; Hwang, Ellen S. ; Amon, Angelika. / Cfi 1 prevents premature exit from mitosis by anchoring Cdc14 phosphatase in the nucleolus. In: Nature. 1999 ; Vol. 398, No. 6730. pp. 818-823.
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