CG5/Dx human breast cancer cell line: Characterization of a new doxorubicin-resistant variant

Nadia Gibelli, Carlo Zibera, Annalia Asti, Luciano Maestri, Luisa Bacchella, Paolo Pedrazzoli, Alberto Calligaro, Gioacchino Robustelli Della Cuna

Research output: Contribution to journalArticlepeer-review

Abstract

By continuous exposure of CG5 human breast cancer cell line to increasing doxorubicin (Dx) concentrations, a multidrug-resistant (MDR) subline (CGS/Dx) was obtained. The resistant variant showed P-glycoprotein (P-gp) expression and a lower intracellular doxorubicin level than the parental cells. CGS/Dx cells were 19.4 fold more resistant to Dx than CG5 cells and showed a cross-resistance to some structurally related and unrelated compounds. Differences in kinetics, biological and ultrastructural features between the two cell lines were investigated. The CGS/Dx cells grew more slowly, produced higher CEA levels and showed a reduced progesterone receptor (PgR) content than the parental cells. Ultrastructural studies revealed differences involving, polyribosomes, rough endoplasmic reticulum, [mithocondria] and cytoskeleton.

Original languageEnglish
Pages (from-to)1675-1681
Number of pages7
JournalAnticancer Research
Volume16
Issue number4 A
Publication statusPublished - Jul 1996

Keywords

  • Breast cancer
  • Doxorubicin
  • Multidrug-resistance

ASJC Scopus subject areas

  • Cancer Research
  • Oncology

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