Change of γδTCR-expressing T cells in healthy aging

Gianluigi Mazzoccoli, G. Vendemiale, A. De Cata, R. Tarquini

Research output: Contribution to journalArticle

Abstract

A mature T-cell lineage with the capacity to proliferate in response to receptor-mediated signals and to display non-major histocompatibility complex (MHC)-restricted cytolysis expresses a CD3-associated heterodimer made up of the protein encoded by the T-cell receptor (TCR) gamma-gene. We investigated the possible differences in lymphocyte subpopulations between healthy young-middle-aged and elderly subjects, focusing attention on γδ-TCR-expressing cells. The study was carried out on fifteen healthy young-middle-aged male subjects (age range 36-55 years) and fifteen healthy elderly male subjects (age range 67-79 years). Lymphocyte subpopulations were analyzed in blood samples collected every four hours for 24 hours. The presence of circadian rhythmicity on absolute counts was validated to evaluate the periodicity of variation, and the fractional variation between single time point values was calculated to evaluate the dynamics of variation. In the group of young and middle-aged subjects a clear circadian rhythm was validated for the time-qualified changes of all the lymphocyte subpopulations (CD3, CD4, CD4/CD8 ratio, CD20, CD25 and HLA-DR with acrophase at night, CD8, CD16 and TcRγδ with acrophase at noon). In the group of elderly subjects a clear circadian rhythm was validated for the nyctohemeral changes of CD3, CD8, CD4/CD8 ratio, CD16, CD25. There was a statistically significant difference for the Midline Estimating Statistic of Rhythm (MESOR) of CD3 (p=0.001), CD25 (p=0.003) and γδ-TCR- expressing cells (p=0.004), higher in the elderly, and for the MESOR of HLA-DR (p=0.002) and CD20 (p=0.002) higher in the young and middle-aged subjects. There was a statistically significant difference between the groups in the fractional variation of TcRγδ-expressing cells between 18:00h and 22:00h values (higher in elderly subjects, p=0.007). In conclusion, specific lymphocyte subsets present different levels and different profiles of nyctohemeral changes in healthy young-middle aged in respect to elderly subjects, since B cells are decreased, whereas CD25 and γδ-TCR-bearing cells are higher in the elderly, but the rhythm and the dynamics of variation of this lymphocyte subset is severely altered and this phenomenon might contribute to the onset of age-related variations of the immune responses.

Original languageEnglish
Pages (from-to)201-209
Number of pages9
JournalInternational Journal of Immunopathology and Pharmacology
Volume24
Issue number1
Publication statusPublished - Jan 2011

Fingerprint

T-Cell Antigen Receptor
Lymphocyte Subsets
T-Lymphocytes
CD4-CD8 Ratio
HLA-DR Antigens
Periodicity
Circadian Rhythm
T-Cell Receptor gamma Genes
Cell Lineage
Major Histocompatibility Complex
Age of Onset
B-Lymphocytes
Proteins

Keywords

  • γδ-TCR expressing T cells
  • Aging
  • Circadian rhythm
  • Lymphocyte subpopulations

ASJC Scopus subject areas

  • Pharmacology
  • Immunology
  • Immunology and Allergy

Cite this

Change of γδTCR-expressing T cells in healthy aging. / Mazzoccoli, Gianluigi; Vendemiale, G.; De Cata, A.; Tarquini, R.

In: International Journal of Immunopathology and Pharmacology, Vol. 24, No. 1, 01.2011, p. 201-209.

Research output: Contribution to journalArticle

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