Change of ganglioside accesibility at the plasma membrane surface of cultured neurons, following protein kinase C activation

Paola Palestini, Marina Pitto, Anita Ferraretto, Guido Tettamanti, Massimo Masserini

Research output: Contribution to journalArticle

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Abstract

While the mechanism of signal transduction across the plasma membrane from the exo- to the endoplasmic side has been extensively investigated, the possible return of messages back to the outer layer is less known. We studied the effect of protein kinase C activation on the ganglioside accessibility at the exoplasmic face of intact rat cerebellar granule cells in culture, using the enzyme sialidase as the probing molecule. Under the experimental conditions (1 milliunit/mL enzyme, 2 min incubation at 37 °C), only GT1b and GD1a gangliosides were partially affected by the enzyme (28.6 and 25.7% hydrolysis, respectively). After cell treatment with phorbol 12-myristate 13- acetate, inducing protein kinase C activation, GT1b and GD1a ganglioside susceptibility to sialidase was strongly decreased (8.6 and 15.9% hydrolysis, respectively). A reduction of ganglioside hydrolysis was also observed when protein kinase C activation was induced by cell treatment for 15 min with 100μM glutamate. On the contrary, accessibility did not vary when protein kinase C translocation was not effective (either in the absence of Ca2+ in the medium or using 1μM glutamate) or when the kinase activity was inhibited by staurosporine. These data suggest that following PKC activation, a key step of inbound transmembrane signaling, cell may dispatch outbound messages to the plasma membrane outer layer, changing the selective recognition and crypticity of glycolipids at the cell surface, possibly through a modulation of their segregation state.

Original languageEnglish
Pages (from-to)3143-3148
Number of pages6
JournalBiochemistry
Volume37
Issue number9
DOIs
Publication statusPublished - Mar 3 1998

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Gangliosides
Cell membranes
Protein Kinase C
Neurons
Chemical activation
Cell Membrane
Hydrolysis
Neuraminidase
Glutamic Acid
Enzymes
Cell signaling
Signal transduction
Staurosporine
Glycolipids
Rats
Signal Transduction
Acetates
Phosphotransferases
Cell Culture Techniques
Modulation

ASJC Scopus subject areas

  • Biochemistry

Cite this

Change of ganglioside accesibility at the plasma membrane surface of cultured neurons, following protein kinase C activation. / Palestini, Paola; Pitto, Marina; Ferraretto, Anita; Tettamanti, Guido; Masserini, Massimo.

In: Biochemistry, Vol. 37, No. 9, 03.03.1998, p. 3143-3148.

Research output: Contribution to journalArticle

Palestini, Paola ; Pitto, Marina ; Ferraretto, Anita ; Tettamanti, Guido ; Masserini, Massimo. / Change of ganglioside accesibility at the plasma membrane surface of cultured neurons, following protein kinase C activation. In: Biochemistry. 1998 ; Vol. 37, No. 9. pp. 3143-3148.
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